FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-38-5-4-1-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-38-5-4-1-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13097
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC392829.99160113002978No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT11698.925708177445218No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC9807.482629609834312No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTG8786.70382530350462No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACC5954.543025120256548No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC2722.07681148354585No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC2682.0462701381995876No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCT1851.4125372222646406No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT1691.2903718408795906No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTAC1681.2827365045430252No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT1591.2140184775139344No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACC1541.1758417958311065No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT1220.9315110330610064No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1190.9086050240513094No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCC1070.816980988012522No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC980.7482629609834314No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC850.6490035886080782No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTACC760.5802855615789876No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGT750.5726502252424219No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTAC710.5421088798961594No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTG650.4962968618767657No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTAC610.4657555165305032No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG590.450484843857372No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC550.41994349851110946No Hit
CGATTCCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACC540.4123081621745438No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTG540.4123081621745438No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTG520.3970374895014126No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG500.3817668168282813No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC500.3817668168282813No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC490.3741314804917157No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG480.36649614415515No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTAC430.3283194624723219No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTGT430.3283194624723219No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA390.2977781171260594No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA390.2977781171260594No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG380.2901427807894938No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT380.2901427807894938No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGT370.28250744445292814No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT370.28250744445292814No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTG340.2596014354432313No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC320.24433076277010002No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTTGGGGTATCTAATCCCAGTGTA300.22906009009696876No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC280.2137894174238375No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT270.2061540810872719No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTACC260.1985187447507063No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC260.1985187447507063No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTACCC250.19088340841414064No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT250.19088340841414064No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAG250.19088340841414064No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTG240.183248072077575No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC230.17561273574100938No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCTCGTGGGGTATCTAATCCCAGT230.17561273574100938No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGT220.16797739940444378No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA210.16034206306787815No Hit
CGATTCCTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGT200.15270672673131253No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC200.15270672673131253No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA200.15270672673131253No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTAA190.1450713903947469No Hit
CGATTCCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTACC190.1450713903947469No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCC190.1450713903947469No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGTA170.12980071772161564No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGTCAGGGGTATCTAATCCCAGTG170.12980071772161564No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTGTA170.12980071772161564No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTG170.12980071772161564No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTA170.12980071772161564No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGTG160.12216538138505001No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGTCGGGGGTATCTAATCCCAG160.12216538138505001No Hit
CGATTCCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT160.12216538138505001No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT160.12216538138505001No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTA150.11453004504848438No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAGT150.11453004504848438No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGG150.11453004504848438No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA150.11453004504848438No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTG150.11453004504848438No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCA140.10689470871191875No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTGTAC140.10689470871191875No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCGTGGGGGTATCTAATCCCAGT140.10689470871191875No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATC140.10689470871191875No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT140.10689470871191875No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT140.10689470871191875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAG157.0778005E-6295.04
TCCTAGA12600.0295.05
TTCCTGA108.369551E-4295.04
TTCCTAG12650.0295.04
ATTCGTA157.0778005E-6295.03
GATTCGT157.0778005E-6295.02
TCGTAGA157.0778005E-6295.05
ATTCCTA12650.0295.03
CCTAGAA12600.0295.06
CGATTCG157.0778005E-6295.01
CGATTCC12850.0293.852141
GATTCCT12800.0293.847662
CTAGAAC12650.0293.833987
AGAACAG13250.0282.75479
TAGAACA13400.0279.589578
CGTAGAA202.2309716E-5221.256
CCTGAAC150.0028176361196.666676
ATTCCTG150.0028176361196.666673
TCCTGAA150.0028176361196.666675
GTAGAAC600.001768922973.757
Produced by FastQC (version 0.11.5)