FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-37-5-4-1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-37-5-4-1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13060
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT373028.560490045941805No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT10518.047473200612558No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT10397.955589586523737No Hit
AGGAATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTGTC7505.742725880551302No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACCCT5183.9663093415007658No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT3202.4502297090352223No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT2842.1745788667687593No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT2011.5390505359877489No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTACCC1941.4854517611026032No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCCTG1931.4777947932618682No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCTGT1921.4701378254211332No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC1651.2633996937212864No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTG1641.2557427258805514No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT1491.1408882082695253No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACCCT1331.0183767228177643No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT1321.010719754977029No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTACCC1270.9724349157733538No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1210.9264931087289433No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT1040.7963246554364471No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTGT780.5972434915773354No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA640.4900459418070444No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGTA630.48238897396630936No Hit
AGGAATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTACCC600.45941807044410415No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT590.45176110260336905No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTACCC580.444104134762634No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTG540.41347626339969373No Hit
AGGAATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTACCCT500.38284839203675347No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT490.3751914241960184No Hit
AGGAATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTACCCT430.329249617151608No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTGT430.329249617151608No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTACCCT410.31393568147013784No Hit
AGGAATAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTGT410.31393568147013784No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTAC390.2986217457886677No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTG370.2833078101071976No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTACCCT370.2833078101071976No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT360.27565084226646247No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTGT350.2679938744257274No Hit
AGGAATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTGT350.2679938744257274No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG330.25267993874425726No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATCCC320.2450229709035222No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTGT310.23736600306278716No Hit
AGGAATAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGTGT310.23736600306278716No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTG280.21439509954058195No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTACC250.19142419601837674No Hit
AGGAATAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTACCCTG250.19142419601837674No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT250.19142419601837674No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAGTGT230.1761102603369066No Hit
AGGAATAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTGTAC230.1761102603369066No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTACC230.1761102603369066No Hit
AGGAATAGAACAGGCTCCTCTAGTCGTCGGGGTATCTAATCCCAGTGTAC230.1761102603369066No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTAATC230.1761102603369066No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGTA230.1761102603369066No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT230.1761102603369066No Hit
AGGAATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTGTA230.1761102603369066No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGTACC220.16845329249617153No Hit
AGGAATAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTGTA200.1531393568147014No Hit
AGGAATAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTGTACCC200.1531393568147014No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG180.13782542113323124No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCCA170.13016845329249618No Hit
AGGAATAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTGT170.13016845329249618No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT170.13016845329249618No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCCTG170.13016845329249618No Hit
AGGAATAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTGTACCC170.13016845329249618No Hit
AGGAATAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTACCC170.13016845329249618No Hit
AGGAATAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGTACC170.13016845329249618No Hit
AGGAATAGAACAGGCTCCTCTAGTCCAGCGTCAGGGGTATCTAATCCCAG160.1225114854517611No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG160.1225114854517611No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAGGTCCTT160.1225114854517611No Hit
AGGAATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTACCCT160.1225114854517611No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACATTGGGGTATCTAATCCCAGT160.1225114854517611No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGGGTATCTAATCCCAGTGTACC150.11485451761102604No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGTAC150.11485451761102604No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTGTACC150.11485451761102604No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAATC150.11485451761102604No Hit
AGGAATAGAACAGGCTCCTCTAGCTTAGGCATCTCGGGGTATCTAATCCC150.11485451761102604No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT150.11485451761102604No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTGTA140.10719754977029097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATAG12850.0293.852142
AGGAATA13050.0289.348661
GAATAGA13050.0289.348663
AACAGGC12850.0289.26079
GAACAGG13000.0285.923078
TAGAACA13050.0284.827586
ATAGAAC13150.0282.66165
AATAGAA13450.0278.550174
AGAACAG13400.0277.388067
AGGAAAG200.006662087147.51
GTATGGG150.0043620459.00000430-34
GGGTTTG452.5465852E-1159.00000425-29
TCCTTAG452.5465852E-1159.00000445-49
GTTTGGG452.5465852E-1159.00000425-29
GGTTGGG150.0043620459.00000420-24
GGTGAAT150.0043620459.00000480-84
GTCATTT150.0043620459.000004215-219
TCTAGTC303.2936077E-759.00000415-19
GGGCACC452.5465852E-1159.00000430-34
TGCCGGA150.0043620459.000004210-214
Produced by FastQC (version 0.11.5)