FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-36-4-4-1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-36-4-4-1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13022
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC232017.816003686069728No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT144111.065888496390723No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTG11959.176777760712639No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC5694.369528490247274No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACC5143.9471663338964826No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCC3762.88742128705268No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACC3722.856704039318077No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTAC3292.526493626171095No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT3122.395945323299032No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCT2922.2423590846260177No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT2431.8660727998771311No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTAC2241.720165873137767No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC1681.2901244048533251No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC1631.2517278451850715No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1311.0059898633082476No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1230.9445553678390416No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC960.7372139456304715No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGT920.7064966978958686No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGTA910.6988173859622178No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTAC890.6834587620949163No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC850.6527415143603132No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTACC820.629703578559361No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT790.6066656427584088No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG760.5836277069574567No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC720.5529104592228536No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAGT650.49915527568729845No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT630.4837966518199969No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTG620.4761173398863462No Hit
CCACGTCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACC570.4377207802180924No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG550.422362156350791No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTTGCCAACAATCCTAGGGGTATCT540.4146828444171402No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTAC530.40700353248348947No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC530.40700353248348947No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTACC530.40700353248348947No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGT510.39164490861618795No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTG500.3839655966825372No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAGT500.3839655966825372No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGTA470.360927660881585No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCA440.33788972508063275No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGT430.330210413146982No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA430.330210413146982No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTG410.3148517892796805No Hit
CCACGTCTAGAACAGGCTCCTCTATGTCTCGTGGGGTATCTAATCCCAGT410.3148517892796805No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC370.28413454154507756No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC350.2687759176777761No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTGTA350.2687759176777761No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGT340.26109660574412535No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGTA320.24573798187682386No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA310.2380586699431731No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAA300.23037935800952233No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCCGCACGTCAGGGGTATCTAATCC290.22270004607587163No Hit
CCACGTCTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCA280.21502073414222087No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGTCGGGGTATCTAATCCCAGTG270.2073414222085701No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGTA260.19966211027491937No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCA250.1919827983412686No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTA250.1919827983412686No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTTGGCTCAGGGGTATCTAA250.1919827983412686No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGT240.18430348640761787No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTACCC240.18430348640761787No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTAATGTGTATAGAGGGGTATCTA240.18430348640761787No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT240.18430348640761787No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTACC220.16894486254031638No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTA220.16894486254031638No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG220.16894486254031638No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCCACGGGGGTATCTAATCCCAGTG220.16894486254031638No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTA210.16126555060666564No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTAC210.16126555060666564No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGCGGGGGTATCTAATCCCAGTG210.16126555060666564No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTGTA210.16126555060666564No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC200.1535862386730149No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTG200.1535862386730149No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTACC190.14590692673936417No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAAT190.14590692673936417No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTG190.14590692673936417No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC170.13054830287206268No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC170.13054830287206268No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA160.12286899093841193No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTGT160.12286899093841193No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGCCGGGGTATCTAATCCCAGT160.12286899093841193No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTACC160.12286899093841193No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC150.11518967900476117No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT150.11518967900476117No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC150.11518967900476117No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTGGGGTATCTAATCCCAGTGTACC140.10751036707111043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTA12800.0295.03
CCACGTC12800.0295.01
CACGTCT12800.0295.02
CGTCTAG12800.0295.04
GTCTAGA12950.0291.5835
TCTAGAA12850.0291.556436
CTAGAAC12950.0289.305027
AGAACAG13450.0278.55029
TAGAACA13800.0271.48558
ACTGCCA150.004361964859.00000435-39
TTGTTGA2500.059.00000490-94
GGGTTTT600.059.00000450-54
GTTGAGG2400.059.000004105-109
CTGAGGC600.059.00000430-34
TCTAGTC150.004361964859.00000420-24
TAGGACT600.059.00000420-24
ATCTTAC150.004361964859.000004170-174
AACTGTT2500.059.000004100-104
GACAGGT150.004361964859.000004185-189
GTTCCCT600.059.00000475-79
Produced by FastQC (version 0.11.5)