FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-35-5-4-1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-35-5-4-1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12745
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC359828.230678697528443No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC10378.13652412710867No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT10037.869752844252647No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTG8006.2769713613181635No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACC3132.455865045115732No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACC2782.181247548058062No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC2772.173401333856414No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTAC2552.000784621420165No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCC2541.9929384072185168No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC2261.7732444095723814No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT2071.624166339741075No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCT2041.6006276971361317No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT1661.302471557473519No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1260.9886229894076108No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC1200.9415457041977247No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTAC1170.9180070615927814No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1130.8866222047861907No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT1120.8787759905845429No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1040.8160062769713613No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA930.7296979207532366No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTAC710.557081208316987No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT600.4707728520988623No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGTA590.4629266378972146No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG550.43154178109062374No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGT540.4236955668889761No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC540.4236955668889761No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC500.3923107100823852No Hit
CCACGTCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACC490.38446449588073756No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC480.37661828167908984No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTACC480.37661828167908984No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTAC470.3687720674774421No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAGT470.3687720674774421No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTAA410.32169478226755593No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTG370.29030992546096507No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT370.29030992546096507No Hit
CCACGTCTAGAACAGGCTCCTCTATGTCTCGTGGGGTATCTAATCCCAGT360.2824637112593174No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG350.2746174970576697No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT340.266771282856022No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC330.25892506865437426No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTG330.25892506865437426No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTACC330.25892506865437426No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTG310.24323264025107885No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC310.24323264025107885No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA310.24323264025107885No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGT300.23538642604943116No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTGTA300.23538642604943116No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTTGCCAACAATCCTAGGGGTATCT280.21969399764613573No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAGT250.1961553550411926No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAA240.18830914083954492No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTA230.1804629266378972No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTACCC220.17261671243624951No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTTGGCTCAGGGGTATCTAA220.17261671243624951No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGT210.1647704982346018No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG210.1647704982346018No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGTA200.1569242840329541No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGT200.1569242840329541No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGTA190.1490780698313064No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTAC180.1412318556296587No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTACC180.1412318556296587No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC180.1412318556296587No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC180.1412318556296587No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGCCGGGGTATCTAATCCCAGT170.133385641428011No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTG170.133385641428011No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGT160.12553942722636327No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC160.12553942722636327No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGCGGGGGTATCTAATCCCAGTG160.12553942722636327No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGTA150.11769321302471558No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCA150.11769321302471558No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTAATGTGTATAGAGGGGTATCTA150.11769321302471558No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC150.11769321302471558No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTGTA150.11769321302471558No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCA150.11769321302471558No Hit
CCACGTCTAGAACAGGCTCCTCTAGACCCTCGATCTCCCTGGGGTATCTA140.10984699882306787No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA140.10984699882306787No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT140.10984699882306787No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCCGCACGTCAGGGGTATCTAATCC140.10984699882306787No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA130.10200078462142016No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTACC130.10200078462142016No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCC130.10200078462142016No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA108.366897E-4294.999974
CCACGTT108.366897E-4294.999971
GTTAGAA108.366897E-4294.999975
CCACGTC12600.0293.829351
CGTCTAG12550.0293.82474
ACGTCTA12700.0291.515753
GTCTAGA12650.0291.501985
CACGTCT12750.0290.372562
TCTAGAA12700.0290.354346
CTAGAAC12850.0286.9657
TAGAACA13200.0278.238658
AGAACAG13500.0272.055549
TTAGAAC150.0028167448196.666676
CACGTTA150.0028167448196.666672
ACGTTAG150.0028167448196.666673
ACGAGAA455.6384044E-498.33333295
TTTGAGT4300.059.00000450-54
GGGTTTT1350.059.00000450-54
CTGAGGC1350.059.00000430-34
GGCGCTT1050.059.00000465-69
Produced by FastQC (version 0.11.5)