FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-34-4-5-7-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-34-4-5-7-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12968
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT234418.075262183837136No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTCA12559.677668106107342No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCTG9006.9401603948180135No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT6595.081739666872301No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCAG5514.248920419494139No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCAGC3913.015114127082048No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATCCA3802.9302899444787167No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTCA3072.3673658235657No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATCCA2632.028069093152375No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT2441.881554595928439No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTCA2401.850709438618137No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCCAG2391.8429981492905614No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT1721.326341764342998No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCAT1541.1875385564466379No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAGCT1541.1875385564466379No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT1411.0872917951881553No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATCCA1250.9639111659469464No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTCA1230.9484885872917952No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTC1080.8328192473781616No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT980.7557063541024059No Hit
ATCTGTAGAACAGGCTCCTCTAGTGTTAGGGGGGTATCTAATCCCAGTCA910.7017273288093769No Hit
ATCTGTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTCA880.6785934608266502No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCATC790.6091918568784701No Hit
ATCTGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCCAG720.5552128315854411No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG710.5475015422578655No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCAT700.5397902529302899No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAATC700.5397902529302899No Hit
ATCTGTAGAACAGGCTCCTCTAGCTTGGGGTATCTAATCCCAGTCATCCA650.5012338062924121No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT640.4935225169648365No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT610.47038864898210975No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCAT580.4472547809993831No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTC560.431832202344232No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCATCCAG530.40869833436150527No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTCAT500.3855644663787785No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGCTCGGGGTATCTAATCCCAGTCAT480.3701418877236274No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTC480.3701418877236274No Hit
ATCTGTAGAACAGGCTCCTCTAGGGTCAGGGGTATCTAATCCCAGTCATC480.3701418877236274No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACTGGGGTATCTAATCCCAGTCATC470.3624305983960518No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCCAG470.3624305983960518No Hit
ATCTGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCAT460.35471930906847626No Hit
ATCTGTAGAACAGGCTCCTCTAGCACGTCAGGGGTATCTAATCCCAGTCA440.3392967304133251No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCAGT440.3392967304133251No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT440.3392967304133251No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCATCC430.3315854410857495No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG430.3315854410857495No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTCATCC410.31616286243059843No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCAG410.31616286243059843No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGTCGGGGTATCTAATCCCAGTCATC400.30845157310302285No Hit
ATCTGTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTCA380.2930289944478717No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCCA370.28531770512029614No Hit
ATCTGTAGAACAGGCTCCTCTAGCATATTGGGGTATCTAATCCCAGTCAT360.27760641579272055No Hit
ATCTGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCATCCA340.26218383713756943No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC330.25447254780999384No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT320.24676125848241826No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTTGGGGTATCTAATCCCAGTC320.24676125848241826No Hit
ATCTGTAGAACAGGCTCCTCTAGCGTAATGTGTATAGAGGGGTATCTAAT320.24676125848241826No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTCAT300.23133867982726714No Hit
ATCTGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCCAG300.23133867982726714No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCTA260.20049352251696484No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGCTCGGGGTATCTAATCCCAGTCA240.1850709438618137No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA240.1850709438618137No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCATCCAG230.17735965453423813No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT220.16964836520666254No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTC220.16964836520666254No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCATCC220.16964836520666254No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTCATCC220.16964836520666254No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTGGGGGTATCTAATCCCAGTCATCC210.16193707587908698No Hit
ATCTGTAGAACAGGCTCCTCTTATAGGGGTATCTAATCCCAGTCATCCAG200.15422578655151142No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTC200.15422578655151142No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTCAT190.14651449722393586No Hit
ATCTGTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCCAGT190.14651449722393586No Hit
ATCTGTAGAACAGGCTCCTCTATAGGGTATCTAATCCCAGTCATCCAGCT190.14651449722393586No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTCATC180.13880320789636028No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCATCCAGC180.13880320789636028No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT170.13109191856878472No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGTGGGGGTATCTAATCCCAGTCAT170.13109191856878472No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAGTCA160.12338062924120913No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCATCC160.12338062924120913No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCATCC160.12338062924120913No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTTCTCGGGGGTATCTAATCCCAGT150.11566933991363357No Hit
ATCTGTAGAACAGGCTCCTCTATGACTACCGGGGTATCTAATCCCAGTCA150.11566933991363357No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT140.107958050586058No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTTGGGGTATCTAATCCCAGTCATCC140.107958050586058No Hit
ATCTGTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTCATCCA140.107958050586058No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTCATC130.10024676125848242No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGTG108.368808E-4294.999971
ATCTGTA12750.0293.843141
CTGTAGA12600.0293.829353
TCTGTAG12700.0292.67722
GAACAGG12500.0292.639988
AACAGGC12500.0291.469
GTAGAAC12700.0289.192935
TGTAGAA12700.0289.192934
AGAACAG13150.0279.296577
TAGAACA13250.0277.18876
GACAGGC150.0028173868196.666668
TCTGTGA150.0028173868196.666662
CTGTGAA150.0028173868196.666663
AGACAGG150.0028173868196.666667
TGTGAAC200.0066614985147.499984
ATCTGAA200.0066614985147.499981
CTCTGGC452.5465852E-1159.00000475-79
TATATTT501.8189894E-1259.00000495-99
TCTAGGC650.059.00000415-19
TGTTCTG2600.059.00000475-79
Produced by FastQC (version 0.11.5)