FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-33-5-4-1-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-33-5-4-1-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12681
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC420133.12830218437032No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC10147.996214809557606No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT10027.901585048497753No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTG6054.770917120100939No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACC3833.020266540493652No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC3132.468259600977841No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC3072.420944720447914No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTAC2341.8452803406671399No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT1881.4825329232710354No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCC1691.332702468259601No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1361.0724706253450043No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCT1321.0409273716583864No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC1311.0330415582367323No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT1240.977840864285151No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTA1240.977840864285151No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1180.9305259837552244No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1070.8437820361170255No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1010.7964671555870988No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG870.6860657676839366No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTG860.6781799542622822No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT800.6308650737323556No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACC760.5993218200457378No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTAC670.5283494992508477No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTAC610.48103461872092107No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTG600.4731488052992667No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC600.4731488052992667No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTACC530.4179481113476855No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA410.3233183502878322No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTG400.3154325368661778No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGT390.3075467234445233No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC390.3075467234445233No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGT370.2917750966012144No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAG340.26811765633625106No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC330.2602318429145966No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGGTCAGGGGTATCTAATCCCAGT320.2523460294929422No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTA310.24446021607128776No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTACC310.24446021607128776No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTG310.24446021607128776No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT310.24446021607128776No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTACC300.23657440264963334No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC280.2208027758063244No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC280.2208027758063244No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT270.21291696238466998No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC270.21291696238466998No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGT260.20503114896301555No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTAC250.1971453355413611No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT230.18137370869805222No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT230.18137370869805222No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG220.17348789527639774No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG210.16560208185474332No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTTGGGGTATCTAATCCCAGTGTAC190.14983045501143444No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATC180.14194464158977999No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTACC180.14194464158977999No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTG170.13405882816812553No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGTA170.13405882816812553No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG170.13405882816812553No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCCA170.13405882816812553No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCCGCACGTCAGGGGTATCTAATCC170.13405882816812553No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA160.1261730147464711No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCA160.1261730147464711No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATTGTCGGGGTATCTAATCCCAGTG160.1261730147464711No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTA160.1261730147464711No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTACC160.1261730147464711No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACGGGGGTATCTAATCCCAGTGTA150.11828720132481667No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGGGTATCTAATCCCAGTGT140.1104013879031622No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTG140.1104013879031622No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT140.1104013879031622No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTCGGGGGTATCTAATCCCAGTGT140.1104013879031622No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC130.10251557448150778No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTCGTGGGGTATCTAATCCCAGTG130.10251557448150778No Hit
GCGAAGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT130.10251557448150778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTT12350.0295.000032
AAGTTAG12250.0295.04
GCGAAGT12600.0293.829351
AGTTAGA12250.0293.795935
GTTAGAA12200.0293.790956
GAAGTTA12400.0292.620973
TTAGAAC12250.0291.387767
TAGAACA13000.0275.711558
AGAACAG13000.0275.711559
CGAAGTA352.0742184E-4126.4285662
GCAGACA550.001251413380.454544295
GGAGATC257.749322E-659.000004195-199
TCCTCTA12300.059.00000415-19
ACCGGGG257.749322E-659.00000430-34
TCCTTAG257.749322E-659.00000445-49
GATACTT257.749322E-659.00000485-89
AACTGTT4700.059.000004100-104
GGCCGTA257.749322E-659.000004210-214
GTTCTCT257.749322E-659.00000475-79
TCGGTAA257.749322E-659.00000495-99
Produced by FastQC (version 0.11.5)