FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-32-6-4-6-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-32-6-4-6-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12576
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT581946.270674300254456No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT9667.681297709923664No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT6264.9777353689567425No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT4633.6816157760814248No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT3162.5127226463104324No Hit
AGGAATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTGT2431.9322519083969467No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTTG1931.5346692111959288No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT1801.4312977099236641No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1321.049618320610687No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT1080.8587786259541985No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGCC1050.8349236641221374No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT980.779262086513995No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT920.7315521628498728No Hit
AGGAATAGAACAGGCTCCTCTGGAGGGATATGAAGCACCGCCAGGTCCTT910.7236005089058524No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAACGCACCGCCAAGTCCTT880.6997455470737913No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCTT870.691793893129771No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTCTGAAGCACCGCCAAGTCCTTT610.48505089058524176No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCTG600.4770992366412214No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT570.4532442748091603No Hit
AGGAATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT530.4214376590330789No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCCTT530.4214376590330789No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCCT470.37372773536895676No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCTT420.33396946564885494No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCATT420.33396946564885494No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG410.3260178117048346No Hit
AGGAATAGAACAGGCTCCTCTATAGGGATATGAAGCACCGCCAGGTCCTT410.3260178117048346No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT410.3260178117048346No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCTT380.30216284987277353No Hit
AGGAATAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCTT380.30216284987277353No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT360.2862595419847328No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGCCCTT350.2783078880407125No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT350.2783078880407125No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGGC340.27035623409669207No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC340.27035623409669207No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTTG300.2385496183206107No Hit
AGGAATAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCTT290.2305979643765903No Hit
AGGAATAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTTGTGG290.2305979643765903No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT240.19083969465648853No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCTT240.19083969465648853No Hit
AGGAATAGAACAGGCTCCTCTGGGTGGGTCTGAGGCACCGCCAAGTCCTT230.1828880407124682No Hit
AGGAATAGAACAGGCTCCTCTAGATGGGGGTATCTAATCCCAGTTGTGGC230.1828880407124682No Hit
AGGAATAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCTT220.17493638676844783No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGACATGAAGCACCGCCAGGTCCTT220.17493638676844783No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTACTT220.17493638676844783No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTC220.17493638676844783No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCTTT220.17493638676844783No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACGGCCAGGTCCTT210.16698473282442747No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGCC210.16698473282442747No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTTGTGGCCT210.16698473282442747No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTCCTT200.1590330788804071No Hit
AGGAATAGAACAGGCTCCTCTAGAGGAATATAAAGCACTGCCAAATCCTC200.1590330788804071No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAATCCTT200.1590330788804071No Hit
AGGAATAGAACAGGCTCCTCTAGAGGTATATGAAGCACCGCCAGGTCCTT190.15108142493638677No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTCCTT180.1431297709923664No Hit
AGGAATAGAACAGGCTCCTCTAGATTATTGAGATTGTGTTTTCCAAAGAC180.1431297709923664No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAGGTCCTT160.1272264631043257No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTTGT160.1272264631043257No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGGCCT160.1272264631043257No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTAATC150.11927480916030535No Hit
AGGAATAGAACAGGCTCCTCTAGATTTCCAAGCATCTCCACCATGCCTCT150.11927480916030535No Hit
AGGAATAGAACAGGCTCCTCTGTCTCTTATAGGGGTATCTAATCCCAGTT150.11927480916030535No Hit
AGGAATAGAACAGGCTCCTCTACAATTCAAGAACCTCGGGATTTCCGGAT150.11927480916030535No Hit
AGGAATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTGGCC140.11132315521628498No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTTGTGGCC140.11132315521628498No Hit
AGGAATAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGTT140.11132315521628498No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTT140.11132315521628498No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGATGTGAAGCACCGCCAAGTCCTT140.11132315521628498No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCTT130.10337150127226462No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCCTT130.10337150127226462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATAG12350.0291.4172
AACAGGC12100.0291.342969
GAACAGG12100.0290.123968
GAATAGA12500.0287.923
AGGAATA12700.0285.708651
AGAACAG12450.0281.967867
ATAGAAC12550.0280.896425
TAGAACA12600.0278.61116
AATAGAA13100.0271.354984
GAGCACG150.0028163472196.66667295
CCATATA150.0028163472196.66667295
GTTGATT6700.059.00000490-94
GTTGATC150.004361260759.000004195-199
GTTGAGG6100.059.000004105-109
GGTTGGC150.004361260759.000004210-214
GTGTTGA150.004361260759.000004195-199
TGGCAAA150.004361260759.00000480-84
TAGTGTT7150.059.00000470-74
CTTTATT150.004361260759.00000490-94
CAGTTAT150.004361260759.000004100-104
Produced by FastQC (version 0.11.5)