FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-31-5-4-1-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-31-5-4-1-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12663
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT372229.392718944957753No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT9637.604832977967306No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT9277.320540156361052No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTGTC6855.40946063334123No Hit
CTAGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACCCT4703.7116007265260995No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT3542.7955460791281688No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT2722.1479902076917003No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT2431.9189765458422177No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC1981.5636105188343994No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACCCT1901.5004343362552317No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTGT1861.468846244965648No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCTGT1561.2319355602937692No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTG1501.1845534233593935No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTACCC1391.097686172313038No Hit
CTAGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCCTG1260.9950248756218906No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTACCC1200.9476427386875147No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT1170.9239516702203269No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT1160.916054647397931No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTGT960.7581141909500119No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT920.726526099660428No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTG900.7107320540156361No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTAC700.552791597567717No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTGT690.544894574745321No Hit
CTAGGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACCCT650.5133064834557372No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTGTA540.42643923240938164No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT500.39485114111979785No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTACCC490.3869541182974019No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT470.37116007265261No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT450.35536602700781805No Hit
CTAGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTACCCT430.33957198136302613No Hit
CTAGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT400.3158809128958383No Hit
CTAGGTAGAACAGGCTCCTCTAGGGTCAGGGGTATCTAATCCCAGTGTAC390.3079838900734423No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTGTA370.29218984442865037No Hit
CTAGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTACCCT360.28429282160625446No Hit
CTAGGTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGTACC350.2763957987838585No Hit
CTAGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTACCC330.2606017531390666No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT320.2527047303166706No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGAGGGGTATCTAATCCCAGTGT320.2527047303166706No Hit
CTAGGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTACCCT320.2527047303166706No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTACC310.2448077074942747No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGTGTA310.2448077074942747No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTGT300.23691068467187867No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAATC300.23691068467187867No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGTA300.23691068467187867No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTACC300.23691068467187867No Hit
CTAGGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT290.22901366184948274No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT290.22901366184948274No Hit
CTAGGTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTGT290.22901366184948274No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG290.22901366184948274No Hit
CTAGGTAGAACAGGCTCCTCTAGCGTCAGACGTGATAAGAGGGGTATCTA290.22901366184948274No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGTAC280.22111663902708678No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT280.22111663902708678No Hit
CTAGGTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTACCCTG280.22111663902708678No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGTA280.22111663902708678No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGTA270.21321961620469082No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTG260.2053225933822949No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTGT250.19742557055989893No Hit
CTAGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTACCCT250.19742557055989893No Hit
CTAGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGTA220.17373450209271105No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTG210.16583747927031509No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTTGGGGGTATCTAATCCCAGTGTA210.16583747927031509No Hit
CTAGGTAGAACAGGCTCCTCTAGTCCACGGGGGTATCTAATCCCAGTGTA200.15794045644791915No Hit
CTAGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGTA200.15794045644791915No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGTGTA190.15004343362552316No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT190.15004343362552316No Hit
CTAGGTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCACG180.14214641080312723No Hit
CTAGGTAGAACAGGCTCCTCTAGTTCTCAGGGGTATCTAATCCCAGTGTA160.1263523651583353No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGTACC160.1263523651583353No Hit
CTAGGTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCCAGT150.11845534233593934No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG150.11845534233593934No Hit
CTAGGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT140.11055831951354339No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT140.11055831951354339No Hit
CTAGGTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTGTACCC140.11055831951354339No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTGTAC140.11055831951354339No Hit
CTAGGTAGAACAGGCTCCTCTTATACAGGGGTATCTAATCCCAGTGTACC140.11055831951354339No Hit
CTAGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTACCCT140.11055831951354339No Hit
CTAGGTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG130.10266129669114744No Hit
CTAGGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTACC130.10266129669114744No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTGACATGGGGTATCTAATCCCAGTG130.10266129669114744No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT130.10266129669114744No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCTT130.10266129669114744No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTGT130.10266129669114744No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGAGGGGTATCT130.10266129669114744No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAA108.3665055E-4295.05
GGTAGAA12250.0295.04
AGGTTAG108.3665055E-4295.03
TAGGTTA108.3665055E-4295.02
CTAGGTA12450.0293.815251
TAGGTAG12350.0292.611362
GAACAGG12350.0291.417028
AGGTAGA12450.0290.261053
AACAGGC12450.0289.07639
TAGAACA12650.0284.505956
GTAGAAC12800.0281.171885
AGAACAG12950.0277.915077
TAGGTAA202.2293472E-5221.252
TTAGAAC150.0028166138196.666666
GGTTAGA200.006659675147.54
GAGCACG200.006659675147.5295
TCAGCAG200.006659675147.5295
CAGCGAA200.006659675147.5295
AGACACG200.006659675147.5295
CTAGGTT300.001354196898.333331
Produced by FastQC (version 0.11.5)