FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-30-4-2-6-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-30-4-2-6-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12959
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT241418.627980554055096No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTTG167812.948529979165057No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTGT11098.557759086349256No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT7976.1501659078632605No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGCC3913.0172081179103327No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTTG3142.4230264680916735No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT3132.415309823288834No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT3062.3612933096689557No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCTG2962.2841268616405586No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT1911.4738791573423875No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGGC1751.3504128404969518No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTT1741.3426961956941121No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCCT1521.1729300100316382No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGCC1461.1266301412145998No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT1210.9337140211436067No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTTG1190.9182807315379272No Hit
ATCTGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGTGGC1100.8488309283123697No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGGC1020.787097769889652No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTTG960.7407979010726137No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTT820.6327648738328574No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG810.6250482290300178No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT810.6250482290300178No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTTG800.617331584227178No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG790.6096149394243383No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTTGT780.6018982946214986No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTGTG770.5941816498186588No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTGGC740.5710317154101396No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTTGT710.5478817810016204No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT690.5324484913959411No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTTGTGG630.48614862257890273No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTT540.41669881935334513No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTTGT530.4089821745505055No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCAGGGGTATCTAATCCCAGTTGTGG490.3781155953391465No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT480.37039895053630684No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTTGT470.3626823057334671No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTTGTG460.35496566093062737No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTGGCC430.3318157265221082No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTTGT400.308665792113589No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTTGTGG400.308665792113589No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGTT390.3009491473107493No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTT380.29323250250790955No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGCTCGGGGTATCTAATCCCA370.2855158577050698No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT340.2623659232965507No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTGGCC330.25464927849371094No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT320.24693263369087123No Hit
ATCTGTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTTGTGGC320.24693263369087123No Hit
ATCTGTAGAACAGGCTCCTCTAGGCACTCGATCTCCTTGGGGTATCTAAT320.24693263369087123No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTTG320.24693263369087123No Hit
ATCTGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTTGTGGCC300.23149934408519174No Hit
ATCTGTAGAACAGGCTCCTCTAGAGCGTCAGGGGTATCTAATCCCAGTTG290.22378269928235206No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTTGTGG290.22378269928235206No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTTG290.22378269928235206No Hit
ATCTGTAGAACAGGCTCCTCTAGGGTAGGGGTATCTAATCCCAGTTGTGG290.22378269928235206No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCATTGGGGTATCTAAT280.2160660544795123No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTGACATTGGGGTATCTAATCCCAGT270.20834940967667256No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG260.20063276487383286No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTT260.20063276487383286No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT260.20063276487383286No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTTGTG240.18519947526815342No Hit
ATCTGTAGAACAGGCTCCTCTAGCTCTCTGTGGGGGTATCTAATCCCAGT230.17748283046531368No Hit
ATCTGTAGAACAGGCTCCTCTAGTTTCGTGGGGTATCTAATCCCAGTTGT230.17748283046531368No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTGGCC230.17748283046531368No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGTGGGGTATCTAATCCCAGTTG230.17748283046531368No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTTGTG210.16204954085963424No Hit
ATCTGTAGAACAGGCTCCTCTAGCGGGGGGTATCTAATCCCAGTTGTGGC200.1543328960567945No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT200.1543328960567945No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTTGTGG190.14661625125395478No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTTGTGG190.14661625125395478No Hit
ATCTGTAGAACAGGCTCCTCTAGATCTCGGGGGTATCTAATCCCAGTTGT190.14661625125395478No Hit
ATCTGTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG190.14661625125395478No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTTGTGG190.14661625125395478No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT180.13889960645111504No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCCCGTGGGGTATCTAATCCCAGTTG180.13889960645111504No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTTGTG170.13118296164827534No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT170.13118296164827534No Hit
ATCTGTAGAACAGGCTCCTCTAGATCTCGTGGGGGTATCTAATCCCAGTT170.13118296164827534No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGTTG160.12346631684543562No Hit
ATCTGTAGAACAGGCTCCTCTAGGGTGGGGGTATCTAATCCCAGTTGTGG150.11574967204259587No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC150.11574967204259587No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGTCGGGGTATCTAATCCCAGTTG150.11574967204259587No Hit
ATCTGTAGAACAGGCTCCTCTAGATCTTGGGGTATCTAATCCCAGTTGTG140.10803302723975615No Hit
ATCTGTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTTGTGGC140.10803302723975615No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCAGTTGT130.10031638243691643No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGCGTCTGGGGTATCTAATCCCAGTT130.10031638243691643No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCACAGTTG130.10031638243691643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTAG12600.0295.02
TCTGCGG108.368808E-4294.999972
ATCTGCG108.368808E-4294.999971
ATCTGTA12850.0292.704281
GAACAGG12550.0292.649388
CTGTAGA12750.0291.529423
GTAGAAC12700.0291.515755
AACAGGC12550.0291.47419
TGTAGAA12750.0290.372564
AGAACAG13000.0283.653877
TAGAACA13100.0281.488566
TCTGTAA202.230575E-5221.249982
CTGTAAA150.0028173868196.666663
TGTAAAA150.0028173868196.666664
TGAGAAT200.0066614985147.49998295
ACTGTTG2600.059.000004100-104
CTCTGGC452.5465852E-1159.00000475-79
GTTGATC257.751396E-659.00000490-94
AGGGGGT257.751396E-659.00000420-24
GAGCGGT257.751396E-659.00000480-84
Produced by FastQC (version 0.11.5)