FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-3-6-5-8-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-3-6-5-8-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10836
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC522048.17275747508305No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC6095.62015503875969No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATC5114.715762273901809No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC4984.595791805094131No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC4173.84828349944629No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC3042.80546327057955No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC2372.187153931339978No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAAT1811.670358065706903No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC1501.3842746400885935No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTC810.7475083056478405No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC720.6644518272425249No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC680.6275378368401624No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTCAA670.6183093392395718No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC630.5813953488372093No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC630.5813953488372093No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTC590.5444813584348468No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG550.5075673680324844No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTC520.47988187523071246No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC500.46142488002953125No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTCAA460.4245108896271687No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC420.3875968992248062No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTC380.35068290882244374No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTC340.3137689184200812No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC330.3045404208194906No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCA320.29531192321889993No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC280.2583979328165375No Hit
TTCTCAGCTAGAACAGGCTCCTCTATCGTGGGGTATCTAATCCCAGTGTC240.22148394241417496No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGAGAGGGGTATCTAATCCCAGTGT240.22148394241417496No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTCA210.1937984496124031No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGAGGGGTATCTAATCCCAGTGTCA210.1937984496124031No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCATATTGGGGTATCTAATCCCAGT210.1937984496124031No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGT190.17534145441122187No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTCA180.16611295681063123No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTC170.1568844592100406No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGAGGGGTATCTAATCCCAGTGT170.1568844592100406No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT170.1568844592100406No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGGGGTATCTAATCCCAGTGT160.14765596160944996No Hit
TTCTCAGCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTG150.13842746400885936No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTC150.13842746400885936No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA150.13842746400885936No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGAGGGGGTATCTAATCCCAGTG140.12919896640826875No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTGGAGGGGTATCTAATCCCAGTG140.12919896640826875No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA130.11997046880767812No Hit
TTCTCAGCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC120.11074197120708748No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC110.10151347360649685No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTC110.10151347360649685No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC110.10151347360649685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCAGC10550.0295.000032
TTCTCAG10650.0295.01
CTCAGCT10600.0293.60853
CAGCTAG10500.0293.595255
TCAGCTA10500.0293.595254
GCTAGAA10400.0293.581737
AGCTAGA10450.0292.177036
CTAGAAC10500.0287.97628
TAGAACA10450.0287.94269
GCAAGAG150.0028109006196.66667295
CTAGAAA150.0028109006196.666678
CTTTTGA150.004357968559.00000450-54
GTAGATG150.004357968559.000004200-204
TTAGAAC150.004357968559.000004115-119
CTCTGGT150.004357968559.00000480-84
GGGTCTA150.004357968559.00000425-29
GTTGAAC150.004357968559.000004100-104
ACTCGGT150.004357968559.000004205-209
ATCTCTG150.004357968559.000004205-209
GGTTGAA150.004357968559.000004100-104
Produced by FastQC (version 0.11.5)