FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-29-4-8-2-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-29-4-8-2-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12893
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT259820.15046924687815No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT135410.50182269448538No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTGT8476.569456294113085No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT7515.824866206468626No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTTGC6525.057007678585279No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT3472.6913829209648648No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT2672.0708911812611497No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCTG2401.8614752191111454No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTTG2371.838206778872256No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT2191.6985961374389205No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTG2151.6675715504537347No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTGCT2031.5744977894981773No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTG2001.551229349259288No Hit
ATCTGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTGTTG1741.3495695338555804No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTGT1591.233227332661134No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT1280.9927867835259443No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTTGC1210.9384937563018692No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTG1160.899713022570387No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTGTT1020.7911269681222368No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT990.7678585278833475No Hit
ATCTGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGTG990.7678585278833475No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTGT930.7213216474055689No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG930.7213216474055689No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT860.6670286201814938No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTGT810.6282478864500116No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGTTG800.6204917397037152No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT750.581711005972233No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGTG700.5429302722407509No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTGT670.5196618320018614No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGTTG610.4731249515240828No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATCCC580.4498565112851935No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTGTG570.4421003645388971No Hit
ATCTGTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCACG480.3722950438222291No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTG470.3645388970759327No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT450.3490266035833398No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGTG430.3335143100907469No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGTTGC420.32575816334445046No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTGTT410.3180020165981541No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTGTT410.3180020165981541No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTGTG410.3180020165981541No Hit
ATCTGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTGTTGC410.3180020165981541No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT340.263708989374079No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAATC340.263708989374079No Hit
ATCTGTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGTGT320.24819669588148607No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACGGGGGTATCTAATCCCAGTG320.24819669588148607No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT320.24819669588148607No Hit
ATCTGTAGAACAGGCTCCTCTAGCATGGGGTATCTAATCCCAGTGTGTTG320.24819669588148607No Hit
ATCTGTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAGTGT310.24044054913518964No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC310.24044054913518964No Hit
ATCTGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTTGC300.23268440238889318No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTGT290.22492825564259675No Hit
ATCTGTAGAACAGGCTCCTCTAGACCATTTCGGGGGGTATCTAATCCCAG270.20941596215000388No Hit
ATCTGTAGAACAGGCTCCTCTAGTCAGCGTCAGGGGTATCTAATCCCAGT250.193903668657411No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT240.18614752191111456No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTG230.17839137516481812No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCGGGGTATCTAATCCCAGTGT220.1706352284185217No Hit
ATCTGTAGAACAGGCTCCTCTAGACTCGTGGGCTCGGAGGGGTATCTAAT200.1551229349259288No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGCTGGGGTATCTAATCCCAGTGT200.1551229349259288No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT190.14736678817963236No Hit
ATCTGTAGAACAGGCTCCTCTAGGGTCGGGGTATCTAATCCCAGTGTGTT190.14736678817963236No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT190.14736678817963236No Hit
ATCTGTAGAACAGGCTCCTCTAGATCTCGGGGTATCTAATCCCAGTGTGT180.1396106414333359No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTGCT180.1396106414333359No Hit
ATCTGTAGAACAGGCTCCTCTAGGGTCTGGGGTATCTAATCCCAGTGTGT180.1396106414333359No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTGTG180.1396106414333359No Hit
ATCTGTAGAACAGGCTCCTCTAGTCCACGGGGGTATCTAATCCCAGTGTG170.1318544946870395No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGTGTT170.1318544946870395No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGCGT170.1318544946870395No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCTA160.12409834794074304No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT160.12409834794074304No Hit
ATCTGTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTGTGTTG160.12409834794074304No Hit
ATCTGTAGAACAGGCTCCTCTAGAGTAAGGGGTATCTAATCCCAGTGTGT160.12409834794074304No Hit
ATCTGTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG160.12409834794074304No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTGTGT150.11634220119444659No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGTGT150.11634220119444659No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTGTTGC150.11634220119444659No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT150.11634220119444659No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT140.10858605444815017No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTGTG140.10858605444815017No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG130.10082990770185372No Hit
ATCTGTAGAACAGGCTCCTCTAGTCATCGACAGCGTCTGGGGTATCTAAT130.10082990770185372No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT130.10082990770185372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGTA12700.0295.01
CTGTAGA12650.0295.03
TCTGTAG12750.0292.686282
AACAGGC12500.0291.469
TGTAGAA12700.0290.354344
GAACAGG12600.0289.146858
GTAGAAC12800.0288.08595
TAGAACA13150.0279.296576
AGAACAG13150.0279.296577
CACGAGG150.0028171325196.66667295
AGAAAGG150.0028171325196.66667295
CGTTTGT257.750716E-659.00000460-64
CTACAGG600.059.00000425-29
ACCGGGG303.2928074E-759.00000425-29
GGGTCTG950.059.00000425-29
GGGTTTT950.059.00000450-54
GGGTCGG150.004361735759.00000420-24
GGCATAG1000.059.000004120-124
TGCCGTA150.004361735759.000004210-214
TCTGGTG150.004361735759.00000475-79
Produced by FastQC (version 0.11.5)