FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-28-4-4-1-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-28-4-4-1-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12598
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT214617.034449912684554No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT127410.112716304175267No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTGTC11028.74742022543261No Hit
CTAGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACCCT6985.540561993967296No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT5694.516589934910304No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT5584.429274488013971No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACCCT2702.143197332909986No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTGT2692.135259565010319No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTG2121.6828067947293222No Hit
CTAGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCCTG2031.6113668836323227No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCTGT2021.6034291157326561No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTACCC1941.539926972535323No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTACCC1911.516113668836323No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT1711.357358310842991No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTG1681.3335450071439912No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTGT1611.277980631846325No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT1581.254167328147325No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTAC970.7699634862676615No Hit
CTAGGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACCCT900.7143991109699952No Hit
CTAGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTACCCT750.595332592474996No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT710.5635815208763295No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTGT700.555643752976663No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTACCC680.5397682171773297No Hit
CTAGGTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGTACC630.5000793776789967No Hit
CTAGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT620.4921416097793301No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTGTA610.4842038418796634No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT610.4842038418796634No Hit
CTAGGTAGAACAGGCTCCTCTAGCGTCAGACGTGATAAGAGGGGTATCTA610.4842038418796634No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGTA590.4683283060803302No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTACC570.45245277028099695No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTGTA570.45245277028099695No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT550.43657723448166375No Hit
CTAGGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTACCCT530.42070169868233054No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTGT520.4127639307826639No Hit
CTAGGTAGAACAGGCTCCTCTAGGGTCAGGGGTATCTAATCCCAGTGTAC480.38101285918399747No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGTA450.3571995554849976No Hit
CTAGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTACCC450.3571995554849976No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGTGTA440.349261787585331No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTACC440.349261787585331No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGTA430.3413240196856644No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC430.3413240196856644No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGAGGGGTATCTAATCCCAGTGT430.3413240196856644No Hit
CTAGGTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTACCCTG420.3333862517859978No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG420.3333862517859978No Hit
CTAGGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT380.3016351801873313No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT360.2857596443879981No Hit
CTAGGTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTGT360.2857596443879981No Hit
CTAGGTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTGTACCC350.2778218764883315No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTG350.2778218764883315No Hit
CTAGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGTA340.26988410858866485No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGTGTA330.26194634068899825No Hit
CTAGGTAGAACAGGCTCCTCTAGTTCTCAGGGGTATCTAATCCCAGTGTA320.25400857278933164No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTG320.25400857278933164No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTGTAC320.25400857278933164No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT310.24607080488966504No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTTGGGGGTATCTAATCCCAGTGTA310.24607080488966504No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTGT300.2381330369899984No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTGT300.2381330369899984No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGTACC290.2301952690903318No Hit
CTAGGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTACC280.2222575011906652No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTGACATGGGGTATCTAATCCCAGTG280.2222575011906652No Hit
CTAGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGTA280.2222575011906652No Hit
CTAGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTACCCT260.20638196539133194No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGTAC250.19844419749166534No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT250.19844419749166534No Hit
CTAGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTACCCT240.19050642959199873No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTG230.18256866169233213No Hit
CTAGGTAGAACAGGCTCCTCTTATACAGGGGTATCTAATCCCAGTGTACC220.1746308937926655No Hit
CTAGGTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG210.1666931258929989No Hit
CTAGGTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCACG210.1666931258929989No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGTACC200.15875535799333226No Hit
CTAGGTAGAACAGGCTCCTCTAGTCCACGGGGGTATCTAATCCCAGTGTA200.15875535799333226No Hit
CTAGGTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCCAGT200.15875535799333226No Hit
CTAGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTACCCT200.15875535799333226No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT180.14287982219399906No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCAGTG170.13494205429433243No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTCGTGGGCTCGGAGATGGGGTATCT170.13494205429433243No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAATC160.12700428639466582No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCAGTGTA160.12700428639466582No Hit
CTAGGTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTGTACC150.1190665184949992No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT140.1111287505953326No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTACC130.10319098269566597No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG130.10319098269566597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGC157.071296E-6295.08
CTAGGTA12450.0295.01
GGTAGAC157.071296E-6295.04
AGGTAGA12500.0293.823
GGTAGAA12350.0293.805664
GAACAGG12400.0292.620978
AACAGGC12350.0292.611339
TAGGTAG12600.0291.48812
GTAGAAC12550.0287.94825
TAGAACA12850.0281.225686
AGAACAG13150.0274.809887
AGACAGG202.228925E-5221.257
TAGACAG255.4272154E-5177.06
AGAGAGG200.006659047147.5295
GTAGACA700.003256779463.2142835
TCCTTTG3350.059.00000445-49
ACTGCCA303.2915523E-759.00000435-39
GGGTTTG303.2915523E-759.00000425-29
GTTTGGG303.2915523E-759.00000425-29
CATGAGC150.004361260759.000004170-174
Produced by FastQC (version 0.11.5)