FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-27-4-2-6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-27-4-2-6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12762
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT193915.193543331766183No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTT128910.100297758971948No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTG11979.379407616361073No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGC6074.756307788747845No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT5674.4428772919605075No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTT2972.32722143864598No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCT2772.1705061902523117No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGG2662.0843128036357936No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT2371.857075693464974No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGG2341.8335684062059237No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTT2261.7708823068484563No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGC2061.6141670584547876No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT2051.6063312960351042No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1901.4887948597398526No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTTG1601.2537219871493497No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCC1541.2067074126312491No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT1331.0421564018178968No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT1060.8305908164864441No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTTG1060.8305908164864441No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTGG1030.8070835292273938No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1020.7992477668077104No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA980.7679047171289767No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTTG970.7600689547092933No Hit
GAGTGGTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCAC870.6817113305124588No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTT850.666039805673092No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT820.6425325184140417No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT800.6268609935746748No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGTGG770.6033537063156246No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTTG700.5485033693778405No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTGT640.5014887948597399No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCC630.4936530324400564No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT630.4936530324400564No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTT600.47014574518100605No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGG540.4231311706629055No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT520.4074596458235386No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTTGTG520.4074596458235386No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGCGGGGTATCTAATCCCAGTTGT500.39178812098417176No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTT480.37611659614480486No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTGGC470.36828083372512144No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTATAGGGGTATCTAATCCCAGTT460.360445071305438No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTTGTG450.3526093088857546No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTTGTG450.3526093088857546No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTTGT420.32910202162670427No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTGGC420.32910202162670427No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTTGTG390.305594734367654No Hit
GAGTGGTAGAACAGGCTCCTCTACTGGGGGTATCTAATCCCAGTTGTGGC380.2977589719479706No Hit
GAGTGGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTGTGGC380.2977589719479706No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCC380.2977589719479706No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT370.2899232095282871No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT360.2820874471086037No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTTG360.2820874471086037No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC350.27425168468892025No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGCTCGGGGTATCTAATCCCAGTT340.2664159222692368No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCAGT320.2507443974298699No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCT300.23507287259050302No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT300.23507287259050302No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT300.23507287259050302No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTTG300.23507287259050302No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTT290.2272371101708196No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT290.2272371101708196No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTTGTG280.21940134775113618No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCCGGGGTATCTAATCC280.21940134775113618No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGTCGGGGGTATCTAATCCCAGT270.21156558533145275No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTTGTG270.21156558533145275No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGCTCGGGGTATCTAATCCCAGTTGT250.19589406049208588No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTT240.18805829807240243No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTCGTGGGCTCGGAGATGGGGTATC230.180222535652719No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTGGC220.17238677323303556No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTCAGATGGTATAAGAGACGGGGTAT220.17238677323303556No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTTGTG190.1488794859739853No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA180.14104372355430184No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGTTCGGAGAGGGGTATC180.14104372355430184No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA170.1332079611346184No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT170.1332079611346184No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTTGTGG170.1332079611346184No Hit
GAGTGGTAGAACAGGCTCCTCTGGGGTATCTAATCCCAGTTGTGGCCTGT150.11753643629525151No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTTGTG150.11753643629525151No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT140.10970067387556809No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT140.10970067387556809No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCAG140.10970067387556809No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGTCGGGGTATCTAATCCCAGTTG130.10186491145588465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA12600.0295.02
GAAAGGC108.3672843E-4295.09
GGTAGAC157.0739607E-6294.999975
TGGTAGA12550.0293.82474
GAGTGGT12750.0292.686281
GGTAGAA12450.0292.630525
GTGGTAG12750.0289.21573
GAACAGG12500.0289.19
GTAGAAC12650.0285.671946
TAGAACA13200.0273.768927
AGAACAG13250.0272.735848
GACAGGC150.0028168755196.666669
AGAAAGG150.0028168755196.666668
TAACAGG150.0028168755196.666669
GTGGTAT150.0028168755196.666663
AGACAGG200.006660292147.58
TCCTTAG257.750024E-659.00000445-49
GGCACCG900.059.00000435-39
TCTAGGC501.8189894E-1259.00000420-24
GACGGGG1000.059.00000420-24
Produced by FastQC (version 0.11.5)