FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-26-5-8-2-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-26-5-8-2-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12559
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC434134.564853889640894No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC134210.685564137272076No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT6485.159646468667887No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCT4843.8538100167210763No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC3532.8107333386416116No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC2872.2852137909069192No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTT2291.8233935822915837No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGT2041.6243331475435945No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT1911.5208217214746398No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1831.457122382355283No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1491.1864001910980173No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT1371.0908511824189824No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC1351.074926347639143No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGC1301.0351142606895454No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTG1170.9316028346205908No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1160.9236404172306713No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTT1070.851978660721395No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT840.6688430607532446No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG810.6449558085834859No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC660.5255195477346922No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC620.4936698781750139No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTG610.48570746078509436No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTG570.45385779122541603No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG570.45385779122541603No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGT520.41404570427581816No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT510.4060832868858986No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT510.4060832868858986No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTG500.398120869495979No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGT420.3344215303766223No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT420.3344215303766223No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTG420.3344215303766223No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAG410.32645911298670277No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG400.3184966955967832No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC390.31053427820686363No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG340.2707221912572657No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC330.2627597738673461No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA330.2627597738673461No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC320.2547973564774265No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGT320.2547973564774265No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC320.2547973564774265No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTG310.24683493908750695No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAA310.24683493908750695No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT300.23887252169758738No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTGT280.22294768691774824No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA270.21498526952782865No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGTT260.20702285213790908No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTA240.19109801735806992No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC230.18313559996815035No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC230.18313559996815035No Hit
GCGAAGTTAGAACAGGCTCCTCTTATACAGGGGTATCTAATCCCAGTGTG210.16721076518831116No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC210.16721076518831116No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT200.1592483477983916No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGT200.1592483477983916No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG200.1592483477983916No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA190.15128593040847202No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCA190.15128593040847202No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGG190.15128593040847202No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGT180.14332351301855245No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGTG180.14332351301855245No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTGGGGTATCTAATC180.14332351301855245No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGGTATCTAATCCCAG170.13536109562863285No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT160.12739867823871326No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCACTCGATCTCATTGGGGTATCTA160.12739867823871326No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC150.11943626084879369No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTT150.11943626084879369No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGT150.11943626084879369No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTCTGGGGTATCTAATCCCAGTGTG140.11147384345887412No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTGT140.11147384345887412No Hit
GCGAAGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT140.11147384345887412No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTGTT130.10351142606895454No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAAT130.10351142606895454No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA130.10351142606895454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAC11850.0295.07
GAAGTTA12200.0295.03
CGAAGTT12200.0295.02
GCGAAGT12300.0295.01
CCGACAA108.3657133E-4295.0295
AAGTTAG12250.0292.591834
GTTAGAA12000.0292.54176
AGTTAGA12300.0290.203255
TAGAACA12550.0280.896428
AGAACAG12600.0278.61119
GTTAGAC202.228925E-5221.256
TTAGACC150.0028163472196.666677
CGACACC150.004361260759.000004280-284
ACCGGGG150.004361260759.00000430-34
AACGCCA150.004361260759.00000435-39
GGGTTTA150.004361260759.000004110-114
AATCTCG150.004361260759.000004200-204
ATCTCGT150.004361260759.000004200-204
TAAATCT150.004361260759.000004200-204
GGGCACC150.004361260759.00000435-39
Produced by FastQC (version 0.11.5)