FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-25-4-4-1-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-25-4-4-1-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12356
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC221117.89414049854322No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT162613.159598575590806No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTG11919.639041761087732No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACC7626.1670443509226285No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC5424.386532858530269No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT2602.1042408546455165No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCT2552.063774684363872No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTAC2421.958562641631596No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT2261.8290708967303333No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACC2011.6267400453221108No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC1711.3839430236322434No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCC1711.3839430236322434No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC1691.3677565555195856No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1271.0278407251537713No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTACC1230.9954677889284558No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTG1110.8983489802525089No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTAC1040.8416963418582066No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTAC980.7931369375202332No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTG970.7850437034639042No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGT810.6555519585626416No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG800.6474587245063127No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG720.5827128520556815No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTG710.5746196179993526No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC710.5746196179993526No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG680.5503399158303658No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA620.5017805114923923No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC600.48559404337973455No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTAC600.48559404337973455No Hit
CGATTCCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACC580.46940757526707677No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT550.44512787309809004No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTGT530.4289414049854322No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTG510.4127549368727744No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGT430.3480090644221431No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC430.3480090644221431No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTG420.3399158303658142No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTTGGGGTATCTAATCCCAGTGTA420.3399158303658142No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT410.3318225963094853No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAG380.30754289414049857No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT380.30754289414049857No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCC350.28326319197151184No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC340.2751699579151829No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGTCGGGGGTATCTAATCCCAG320.2589834898025251No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC310.25089025574619617No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA300.24279702168986728No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTG300.24279702168986728No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTACCC290.23470378763353839No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGTA290.23470378763353839No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGTCAGGGGTATCTAATCCCAGTG290.23470378763353839No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTACC290.23470378763353839No Hit
CGATTCCTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGT280.22661055357720947No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA280.22661055357720947No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTAC280.22661055357720947No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAGT240.19423761735189382No Hit
CGATTCCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTACC240.19423761735189382No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCTCGTGGGGTATCTAATCCCAGT240.19423761735189382No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCGTGGGGGTATCTAATCCCAGT230.1861443832955649No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGTGGGGTATCTAATCCCAGTGTAC230.1861443832955649No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGG230.1861443832955649No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT230.1861443832955649No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGTG220.178051149239236No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC220.178051149239236No Hit
CGATTCCTAGAACAGGCTCCTCTTATAGGGGTATCTAATCCCAGTGTACC220.178051149239236No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTGGGGTATCTAATCCCAGTGTACC220.178051149239236No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC210.1699579151829071No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTA210.1699579151829071No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTGTAC210.1699579151829071No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA210.1699579151829071No Hit
CGATTCCTAGAACAGGCTCCTCTAGATCTGGGGTATCTAATCCCAGTGTA210.1699579151829071No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTA210.1699579151829071No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTGTA200.16186468112657817No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCA180.14567821301392037No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT180.14567821301392037No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA180.14567821301392037No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTG180.14567821301392037No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACTGGGGTATCTAATCCCAGTGT170.13758497895759145No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC170.13758497895759145No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTCTGGGGGTATCTAATCCCAGTG170.13758497895759145No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGT160.12949174490126256No Hit
CGATTCCTAGAACAGGCTCCTCTATACGGGGTATCTAATCCCAGTGTACC160.12949174490126256No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGT160.12949174490126256No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGCGGGGTATCTAATCCCA150.12139851084493364No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATC150.12139851084493364No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC140.11330527678860473No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC140.11330527678860473No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGT140.11330527678860473No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTCTAGGGAGACAGGGGGTATCTAA130.10521204273227583No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTAG108.364092E-4295.04
TTCCTAG11950.0295.04
GATTCCT12050.0295.02
ATTCCTA12000.0295.03
CTAGACC108.364092E-4295.07
AGACCAG108.364092E-4295.09
CCTAGAA11800.0295.06
CGATTCC12100.0295.01
CGATTAC108.364092E-4295.01
TCCTAGA12000.0293.77085
CTAGAAC12100.0288.904977
TAGAACA12450.0279.59848
AGAACAG12600.0276.269849
CCTAGAC202.2280607E-5221.256
GATTACT150.0028158033196.666662
TAGACCA150.0028158033196.666668
TCCTCTA12150.059.00000415-19
GGGTTTT452.5465852E-1159.00000450-54
CAGTTTT2250.059.00000485-89
AGGGCTA3100.059.000004115-119
Produced by FastQC (version 0.11.5)