FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-24-5-8-2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-24-5-8-2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12418
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC334726.9528104364632No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC11349.131905298759865No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT10208.213883072958609No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCT6004.83169592526977No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT4773.8411982605894672No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTT3302.657432758898373No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC3262.625221452729908No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC2832.278949911418908No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA2552.0534707682396522No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT2391.9246255435657917No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGC1941.562248349170559No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT1881.5139313899178612No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1271.022708970848768No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTT1251.0066033177645353No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTG1130.90996939925914No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1120.9019165727170236No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGT1080.8697052665485585No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1070.8616524400064423No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1040.8374939603800934No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC970.7811241745852795No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGTT770.6200676437429538No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG750.6039619906587212No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT730.5878563375744886No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGT640.5153808986954421No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGT530.42679980673216306No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC460.370430020937349No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTG460.370430020937349No Hit
CCACGTCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTGTT430.3462715413110002No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTG430.3462715413110002No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC410.33016588822676757No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTGTT400.3221130616846513No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTGT370.2979545820583025No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGT360.2899017555161862No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC330.2657432758898373No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTG320.25769044934772106No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA320.25769044934772106No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGT310.24963762280560478No Hit
CCACGTCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTT290.2335319697213722No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTG280.2254791431792559No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTGTT260.20937349009502335No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGT260.20937349009502335No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCC260.20937349009502335No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTG240.19326783701079078No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG240.19326783701079078No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT220.17716218392655822No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTGT220.17716218392655822No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAGT220.17716218392655822No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTG210.16910935738444194No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC210.16910935738444194No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA210.16910935738444194No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT200.16105653084232566No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT190.15300370430020938No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATC190.15300370430020938No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATC180.1449508777580931No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT180.1449508777580931No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTTGGGGTATCTAATCCCAG180.1449508777580931No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACTCGGGTATCTAATCCCAGT180.1449508777580931No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTGTT170.1368980512159768No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC160.12884522467386053No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGT160.12884522467386053No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTG160.12884522467386053No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC160.12884522467386053No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA150.12079239813174425No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGTCAGGGGTATCTAATCCCAGTGT150.12079239813174425No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC150.12079239813174425No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTG140.11273957158962795No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT140.11273957158962795No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGT130.10468674504751167No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTG130.10468674504751167No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC130.10468674504751167No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGGC108.364502E-4295.01
CACGTCT12100.0295.02
CAAGCTA108.364502E-4295.0295
CGTCTAG12050.0295.04
CCACGTC12150.0293.785981
ACGTCTA12100.0293.7813
CTAGAAC12050.0293.77597
GTCTAGA12150.0292.5725
TCTAGAA12100.0292.561986
TAGAACA12400.0285.483868
AGAACAG12550.0282.071729
TACTAGG150.0028159407196.66667295
CGTTTGT303.2908974E-759.00000460-64
GTTTGTG303.2908974E-759.00000465-69
TTGTTGA4050.059.00000490-94
GAGCGGT501.8189894E-1259.00000485-89
CAGTTTT3750.059.00000485-89
ATCCAAG150.004361015359.000004140-144
AACTGTT4300.059.000004100-104
CCAGTGG150.004361015359.000004145-149
Produced by FastQC (version 0.11.5)