FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-23-4-8-2-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-23-4-8-2-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12563
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC249319.843986309002627No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT153112.18657963862135No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCT12019.559818514685983No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTT6475.150043779352066No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC6144.887367666958529No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT4273.398869696728488No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGC3022.403884422510547No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT2451.9501711374671655No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGT2161.719334553848603No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC2051.6317758497174242No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC1881.4964578524237842No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC1591.2656212688052217No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTG1561.2417416222239912No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTG1541.2258218578365039No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTGT1281.0188649207991722No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1240.987025392024198No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGT1090.8676271591180451No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1080.8596672769243014No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1070.8517073947305579No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTT960.7641486905993792No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC940.7482289262118921No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG840.6686301042744567No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC790.628830693305739No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGTT760.6049510467245085No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG730.581071400143278No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC700.5571917535620472No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGT600.47759293162461197No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTGTT600.47759293162461197No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGT590.4696330494308685No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAG580.46167316723712487No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG560.4457534028496378No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTG490.39003422749343314No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTGT420.33431505213722834No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC390.3104354055559978No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAG370.2945156411685107No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGTGGGGTATCTAATCCCAGTGT350.2785958767810236No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTG350.2785958767810236No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC340.2706359945872801No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTG340.2706359945872801No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTG330.2626761123935366No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT310.2467563480060495No Hit
CGATTCCTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCCA300.23879646581230599No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTGAGGGGTATCTAATCCCAGTGT280.2228767014248189No Hit
CGATTCCTAGAACAGGCTCCTCTAGTAGCGTCAGGGGTATCTAATCCCAG270.21491681923107536No Hit
CGATTCCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTGTT250.19899705484358832No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTGT250.19899705484358832No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGTG240.1910371726498448No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC240.1910371726498448No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGT230.18307729045610124No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT220.17511740826235772No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGT210.16715752606861417No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC200.15919764387487065No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC200.15919764387487065No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG200.15919764387487065No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGT200.15919764387487065No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC200.15919764387487065No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTTGGCGGGGTATCTAATCCCAGT180.14327787948738357No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTCTGGGCTCGGAGGGGTATCTAA180.14327787948738357No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA180.14327787948738357No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTG170.13531799729364005No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC160.12735811509989653No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA160.12735811509989653No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGC160.12735811509989653No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC160.12735811509989653No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCTGAGGGGTATCT160.12735811509989653No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTA140.11143835071240946No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT140.11143835071240946No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAGT140.11143835071240946No Hit
CGATTCCTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTGTGT140.11143835071240946No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTGT130.10347846851866593No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGGTATCTAATCCCAGTGTGT130.10347846851866593No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTGTG130.10347846851866593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTAG12350.0295.04
GATTCCT12450.0295.02
ATTCCTA12400.0295.03
TCCTAGA12400.0293.81055
CGATTCC12550.0292.64941
CTAGAAC12400.0292.620977
CCTAGAA12400.0292.620976
TAGAACA12950.0281.332038
AGAACAG13050.0278.0469
ACACGAG200.006659047147.5295
CACGATA455.636824E-498.33333295
GCCCTCG150.004361260759.00000425-29
TGTTGAA303.2915523E-759.000004105-109
GGGTTTG150.004361260759.00000425-29
GTTTGGG150.004361260759.00000430-34
GTCATTA150.004361260759.000004220-224
GTGTCAT150.004361260759.000004215-219
CTTTATA150.004361260759.00000425-29
GGCACCG950.059.00000435-39
GGGCACC150.004361260759.00000435-39
Produced by FastQC (version 0.11.5)