FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-220-6-5-8-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-220-6-5-8-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621115
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC29437747.3949268653953No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC353015.68348856491954No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC285694.599631308211845No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATCA273754.407396375872423No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC147322.3718635035379925No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC134632.1675535126345364No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC128652.0712750456839717No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC118851.91349428044726No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC102471.649775001408757No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC92301.4860372072804553No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC73301.1801357236582597No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAATC68711.1062363652463714No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCAA64151.0328200091770445No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT53160.8558801510187324No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTCAA52940.852338133839949No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCC51960.836560057316278No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC46270.744950613010473No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT45750.7365785724060762No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA43550.701158400618243No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT34580.556740700192396No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTCAAT27530.44323514969047595No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC25130.4045949622855671No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTG25050.4033069560387368No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTG24580.3957399193386088No Hit
CGATTCCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGT18270.2941484266198691No Hit
CGATTCCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCC17390.2799803579047358No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC14180.22829910725067018No Hit
CGATTCCTAGAACAGGCTCCTCTAGGAATCGCTGGGGGTATCTAATCCCA14040.22604509631871714No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATATAAAGCACTGCCAAGTCC13750.22137607367395734No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTCAA13510.21751205493346643No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTTGCCAACAATCCTAGGGGTATCT13390.21558004556322097No Hit
CGATTCCTAGAACAGGCTCCTCTAGACCACGAGACAGGGGTATCTAATCC12650.20366598778004072No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTCAAT12550.20205597997150285No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAG12550.20205597997150285No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGT11810.1901419221883226No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC11070.17822786440514238No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA10690.17210983473269845No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCC10600.17066082770501437No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATATAAAGCACCGCCAAGTCC10480.1687288183347689No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGT10320.16615280584110834No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTC10150.16341579256659394No Hit
CGATTCCTAGAACAGGCTCCTCTAGGAATCGTCTTGGGGTATCTAATCCC9290.14956972541316826No Hit
CGATTCCTAGAACAGGCTCCTCTAGGAAGGGGTATCTAATCCCAGTGTCA9060.14586670745353117No Hit
CGATTCCTAGAACAGGCTCCTCTGGAGGGATATGAAGCACCGCCAGGTCC8430.13572365825974256No Hit
CGATTCCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT7190.11575956143387296No Hit
CGATTCCTAGAACAGGCTCCTCTACGAGACCACGAGACGGGGTATCTAAT7070.1138275520636275No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC7040.11334454972106614No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAGGTCC6970.11221754425508963No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATGTGAAGCACCGCCAGGTCC6970.11221754425508963No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTCAA6720.10819252473374497No Hit
CGATTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT6500.10465050755496164No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC6420.10336250130813134No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGATATGAAGCACCGCCAGGTCCT6300.1014304919378859No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTAG607950.0294.951484
GATTCCT613050.0294.903752
TCCTAGA607750.0294.854375
ATTCCTA610700.0294.830933
CGATTCC615550.0294.80831
CCTAGAA606250.0294.464726
CTAGAAC611300.0291.766757
TAGAACA630050.0283.200968
AGAACAG632750.0281.75949
TTCCTGA1900.0263.947364
GATTCCC400.0258.1252
CGATTCG1150.0256.521731
TCCTGGA351.2732926E-11252.857135
ATTCCTG2400.0245.833343
CGATTCT2200.0241.363631
GATTCTA1800.0237.63892
GATTCCA2050.0237.439012
TTCCTAA2850.0232.894744
TTCCAGA1750.0227.571434
CGATTTC1300.0226.923081
Produced by FastQC (version 0.11.5)