FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-22-4-2-6-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-22-4-2-6-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12414
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT247419.9291122925729No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTTG133610.762042854841308No Hit
AGGAATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTGT9487.63653939101015No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT7415.96906718221363No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGCC5024.0438214918640245No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCTG3542.8516191396810053No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTTG3242.609956500724988No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT2982.400515546963106No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT2612.1024649589173516No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGGC2481.9977444820364103No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT2331.8769131625584017No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTT1811.4580312550346384No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTT1521.2244240373771549No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTTG1481.1922023521830192No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCCT1170.9424842919284679No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT1150.9263734493314No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1100.8860963428387304No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGCC1020.8216529724504592No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT840.6766553890768487No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTGTG750.6041565973900435No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTT740.5961011760915096No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG730.5880457547929757No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAATC620.4994361205091026No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTTG590.4752698566135009No Hit
AGGAATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTTGTGGCC580.467214435314967No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT550.4430481714193652No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTTGT520.4188819075237635No Hit
AGGAATAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTTG480.3866602223296278No Hit
AGGAATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGTGGC470.37860480103109395No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT450.3624939584340261No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT440.35443853713549217No Hit
AGGAATAGAACAGGCTCCTCTAGTCGTCGGGGTATCTAATCCCAGTTGTG430.3463831158369583No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTGGCC410.33027227323989045No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT400.3222168519413565No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTGGC400.3222168519413565No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGGC380.3061060093442887No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGTT360.28999516674722087No Hit
AGGAATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTTGT350.28193974544868694No Hit
AGGAATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTTG340.27388432415015307No Hit
AGGAATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTGGCC330.26582890285161914No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA320.2577734815530852No Hit
AGGAATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTGGCC320.2577734815530852No Hit
AGGAATAGAACAGGCTCCTCTAGTCCCGTGGGGTATCTAATCCCAGTTGT320.2577734815530852No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTGGCC290.2336072176574835No Hit
AGGAATAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTTGTGGC280.2255517963589496No Hit
AGGAATAGAACAGGCTCCTCTAGTTGCAGCAGGGGTATCTAATCCCAGTT280.2255517963589496No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTTGTGG280.2255517963589496No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAATC280.2255517963589496No Hit
AGGAATAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTTGTG270.21749637506041566No Hit
AGGAATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTGGCC270.21749637506041566No Hit
AGGAATAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTTGTGGC270.21749637506041566No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT270.21749637506041566No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGTTG250.20138553246334784No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAGT240.1933301111648139No Hit
AGGAATAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTGTGGCC240.1933301111648139No Hit
AGGAATAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGTTG230.18527468986628No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTTGT220.17721926856774609No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT220.17721926856774609No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTAAT220.17721926856774609No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT210.1691638472692122No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTTGT200.16110842597067826No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTGGGCTCGGGGTATCTAATCCC190.15305300467214436No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCCTGGGGTATCTAAT190.15305300467214436No Hit
AGGAATAGAACAGGCTCCTCTAGCAGTTCCATGGGGTATCTAATCCCAGT180.14499758337361043No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGGCCT180.14499758337361043No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTTGT180.14499758337361043No Hit
AGGAATAGAACAGGCTCCTCTAGCTCTCGTGGGGTATCTAATCCCAGTTG180.14499758337361043No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT170.13694216207507653No Hit
AGGAATAGAACAGGCTCCTCTGGGGTATCTAATCCCAGTTGTGGCCTGTC170.13694216207507653No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG170.13694216207507653No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC170.13694216207507653No Hit
AGGAATAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTTGTGGCCT160.1288867407765426No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTTGTGG160.1288867407765426No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTT160.1288867407765426No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTTG160.1288867407765426No Hit
AGGAATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTTGT150.1208313194780087No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT150.1208313194780087No Hit
AGGAATAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTTGTGG150.1208313194780087No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG150.1208313194780087No Hit
AGGAATAGAACAGGCTCCTCTAGACATTGGGGTATCTAATCCCAGTTGTG150.1208313194780087No Hit
AGGAATAGAACAGGCTCCTCTAGACGTCAGGGGTATCTAATCCCAGTTGT140.1127758981794748No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG140.1127758981794748No Hit
AGGAATAGAACAGGCTCCTCTAGATCTCGTGGGCTCGGGGTATCTAATCC130.10472047688094087No Hit
AGGAATAGAACAGGCTCCTCTAGCTAAGCTTGGAACAGGGCGGGGGGTAT130.10472047688094087No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAATG108.364502E-4295.01
AGGAATA12100.0295.01
AGCAGGC108.364502E-4295.09
GGAATGA108.364502E-4295.02
GGAATAG12100.0295.02
GGTATGG108.364502E-4295.0295
AACAGGC11800.0291.259
GAACAGG11900.0288.802528
GAATAGA12300.0287.804873
ATAGAAC12500.0279.665
AGAACAG12350.0279.47377
TAGAACA12500.0277.36
AATAGAA12800.0273.105474
GAGCAGG150.0028159407196.666678
ATAGAGC301.1220656E-4147.55
GAACAGC200.006658088147.58
AGAGCAG403.5274215E-4110.6257
AATAGAG403.5274215E-4110.6254
TAGAGCA403.5274215E-4110.6256
GCCCTCG303.2908974E-759.00000420-24
Produced by FastQC (version 0.11.5)