FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-219-6-5-8-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-219-6-5-8-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432215
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT12173428.16514928912694No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATCACT5364112.41072151591222No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT236895.480837083395995No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAATCAC182344.218733732054649No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT165383.8263364297861022No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCAATC109772.5397082470529715No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCTT109522.5339240887058523No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT90282.088775262311581No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT88232.041345163865206No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT70681.6352972478974586No Hit
ATCTGTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCTT58071.3435443008687806No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTCAATCA42100.9740522656548246No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT41590.962252582626702No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT38490.8905290191224275No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT35720.8264405446363499No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG30990.7170042687088601No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGTC26560.6145089827979131No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT21140.48910842983237507No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT20470.4736068854620964No Hit
ATCTGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTCAATC19600.4534780144141226No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTG19100.4419096977198848No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTCAAT18920.43774510370995917No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTGT16490.38152308457596334No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT16050.37134296588503407No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTCAATC15490.3583864511874877No Hit
ATCTGTAGAACAGGCTCCTCTAGGGTATCTAATCCCAGTGTCAATCACTG14710.3403398771444767No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT14640.33872031280728343No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTG14050.32506969910808275No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT14030.3246069664403133No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC13720.3174346100898858No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT13570.3139641150816145No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCTT13340.3086426894022651No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT13310.3079485904006108No Hit
ATCTGTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCTTTG12730.29452934303529493No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTG12560.2905961153592541No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTCAATC12010.2778709669955925No Hit
ATCTGTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTCAA11890.2750945709889754No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTCAAT11700.27069861064516504No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTCAATCA11490.26583991763358517No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT10800.24987564059553696No Hit
ATCTGTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGTCA10650.24640514558726562No Hit
ATCTGTAGAACAGGCTCCTCTACAGATGGGGTATCTAATCCCAGTGTCAA10210.23622502689633632No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTGTCA9780.22627627453929178No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTGT9760.22581354187152228No Hit
ATCTGTAGAACAGGCTCCTCTAACGGGGGGTATCTAATCCCAGTGTCAAT9680.22396261120044425No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGTG9500.2197980171905186No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT9400.21748435385167106No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT9070.2098492648334741No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGACGACGAGGGGTATCTAATCCCAG8930.20661013615908747No Hit
ATCTGTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGTGT8790.2033710074847009No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTGTCA8620.19943777980866007No Hit
ATCTGTAGAACAGGCTCCTCGTCTCGTGGGGTATCTAATCCCAGTGTCAA8590.1987436808070058No Hit
ATCTGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTCAATC8260.19110859178880882No Hit
ATCTGTAGAACAGGCTCCTCTAGACGACGAGACGGGGTATCTAATCCCAG8140.18833219578219174No Hit
ATCTGTAGAACAGGCTCCTCTGGTCTCGTGGTCTCGGGGTATCTAATCCC8140.18833219578219174No Hit
ATCTGTAGAACAGGCTCCTCTAGACGCTGCCGAGAGGGGTATCTAATCCC8100.1874067304466527No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGAGAACCAAGGAAAGGTTATTACAC7930.18347350277061186No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTCAATC7480.1730620177457978No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGTT7230.1672778593986789No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGTCAA7180.1661210277292551No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTCCTT7070.16357599805652281No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT7030.16265053272098376No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCTT6970.16126233471767523No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGGGGTATCTAATCCCAGTGTCAA6950.1607996020499057No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCTT6900.15964277038048194No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACTCGGGGGTATCTAATCCCAGTG6710.15524681003667157No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCTT6630.1533958793655935No Hit
ATCTGTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCCAGT6540.1513135823606307No Hit
ATCTGTAGAACAGGCTCCTCGACGAGGGGTATCTAATCCCAGTGTCAATC6490.15015675069120693No Hit
ATCTGTAGAACAGGCTCCTCTAGCCACGAGAGGGGTATCTAATCCCAGTG6390.14784308735235935No Hit
ATCTGTAGAACAGGCTCCTCTAGAGAGAGACGAGACGGGGTATCTAATCC6110.14136483000358618No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTTT6110.14136483000358618No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCTT6050.13997663200027766No Hit
ATCTGTAGAACAGGCTCCTCTAGACGAGACGGGTATCTAATCCCAGTGTC5990.1385884339969691No Hit
ATCTGTAGAACAGGCTCCTCTAGAGAGAGAGGGGTATCTAATCCCAGTGT5640.13049061231100262No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCTT5640.13049061231100262No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT5630.13025924597711788No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATGTGAAGCACCGCCAAGTCCTT5600.12956514697546362No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATACGAAGCACCGCCAGGTCCTT5510.12748284997050077No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCCT5320.12308688962669043No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTCAA5250.12146732528949714No Hit
ATCTGTAGAACAGGCTCCTCTTCGTCGGGGTATCTAATCCCAGTGTCAAT5110.11822819661511054No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCCTT4970.11498906794072394No Hit
ATCTGTAGAACAGGCTCCTCTAGCCCACAGGGGTATCTAATCCCAGTGTC4690.10851081059195078No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCCTT4660.1078167115902965No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCTA4660.1078167115902965No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGAGGGGTATCTAATCCCAGTGTCAA4660.1078167115902965No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCAGGGGTATCTAATCCCAGTGTCAA4650.10758534525641174No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGTC4560.10550304825144893No Hit
ATCTGTAGAACAGGCTCCTCTAGACGAGGGGTATCTAATCCCAGTGTCAA4550.10527168191756418No Hit
ATCTGTAGAACAGGCTCCTCGGGGGGGGTATCTAATCCCAGTGTCAATCA4530.10480894924979466No Hit
ATCTGTAGAACAGGCTCCTCTAGCCCACGAAGGGGTATCTAATCCCAGTG4520.1045775829159099No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGACATGAAGCACCGCCAGGTCCTT4480.10365211758037088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTAG427700.0294.17232
ATCTGTA431550.0293.01761
CTGTAGA429700.0292.597173
GAACAGG423850.0291.6948
AACAGGC424800.0290.97229
GTAGAAC432300.0287.561865
TGTAGAA435650.0287.483644
TAGAACA440850.0281.18186
AGAACAG440800.0280.845647
AACGGCT450.0229.444449
GAACGGC600.0196.666678
GAAAGGC1900.0178.552638
AACCGGC750.0177.09
GAACCGG750.0177.08
GACAGGC1900.0163.02638
AACGGGC401.9555555E-6147.59
AAAGGCT2600.0141.826929
TCTGTAA4100.0122.3170852
TCTGTGA2500.0118.000012
TCTGAGA3150.0112.380952
Produced by FastQC (version 0.11.5)