FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-218-6-5-7-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-218-6-5-7-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342445
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC16642348.59846106674065No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC233056.805472411628144No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC141244.124457942151294No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC136693.9915898903473552No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGC114263.3365941976083753No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC79232.3136562075661784No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC76972.2476602082086172No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC76562.2356874826614495No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAG61571.7979529559491305No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC57191.670049204981822No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCC50351.4703091007315043No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT38331.1193038298120865No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT32620.9525617252405496No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC30340.8859816904904437No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC23740.6932500109506636No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT22430.65499569273898No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCC18050.5270919417716713No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC14400.4205054826322475No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTAC13330.3892595891311013No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCATCCA13300.38838353604228415No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC12570.36706624421439943No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTCATCCA11450.33436026223189125No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCT10920.3188833243294544No Hit
CGATTCCTAGAACAGGCTCCTCTTACAGGGGTATCTAATCCCAGTCATCC10830.31625516506300283No Hit
CGATTCCTAGAACAGGCTCCTCTAGGAATCGCTGGGGGTATCTAATCCCA9740.2844252361693118No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT9570.2794609353326812No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG6650.19419176802114207No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC6610.1930236972360525No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA6280.1833871132590635No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC6090.177838777029888No Hit
CGATTCCTAGAACAGGCTCCTCTAGGAATCGTGGGGTATCTAATCCCAGT5910.17258245849698492No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAG5910.17258245849698492No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCT5670.16557403378644747No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCC5470.1597336798609996No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTCAT5450.15914964446845478No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATATAAAGCACCGCCAAGTCC5010.14630086583246946No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATATAAAGCACTGCCAAGTCC4780.1395844588182044No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTATGAGGCACCGCCAAGTCC4650.13578822876666327No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCC4500.13140796332257737No Hit
CGATTCCTAGAACAGGCTCCTCTAGACCAGACGGGGTATCTAATCCCAGT4190.12235541473813313No Hit
CGATTCCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT4120.12031129086422637No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC3970.11593102542014047No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC3880.11330286615368892No Hit
CGATTCCTAGAACAGGCTCCTCTGGAGGGATATGAAGCACCGCCAGGTCC3880.11330286615368892No Hit
CGATTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT3530.10308224678415512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAG206.178561E-8295.04
TTCCTGA700.0295.04
TTCCTAG335350.0295.04
ATTCCTA336550.0294.86853
CGATTCC338600.0294.825741
GATTCCT337400.0294.825132
TCCTAGA335450.0294.736185
CCTAGAA333600.0294.602086
CTAGAAC336250.0292.01717
AGAACAG346600.0283.254469
TAGAACA347150.0283.23068
TTCCTAA950.0279.473664
ATTCTAG800.0276.56253
TTCCAGA550.0268.181824
CGATTCG600.0245.833311
CGATTCT1450.0244.137941
TTTCTAG500.0236.04
ATTCCTG950.0232.894733
GATTCCC202.2807244E-5221.252
CCTGAAC800.0221.256
Produced by FastQC (version 0.11.5)