FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-217-6-1-2-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-217-6-1-2-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323001
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT13235340.976034129925296No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT248697.699356967935084No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTG167225.177073755189612No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT121963.7758397032826525No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT117363.633425283513054No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT113573.5160881854854944No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT60341.868105671499469No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT54131.6758462048105116No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT37031.146436079145266No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT31290.9687276509979845No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT28980.8972108445484689No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCT28690.88823254417169No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG28300.8761582781477456No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT26180.8105238064278439No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT20000.6191931294330358No Hit
TGCTTATAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTGT17200.5325060913124108No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA16820.5207414218531831No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTGTTG15510.4801842718753193No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT15250.47213476119268977No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCT14740.4563453363921474No Hit
TGCTTATAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTTG13640.4222897142733304No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTGC13510.4182649589320157No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT13140.40680988603750456No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTT12710.39349723375469425No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTTG11050.3421042040117523No Hit
TGCTTATAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGTG10340.3201228479168795No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTCCT10210.31609809257556476No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT9820.3040238265516206No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT9230.285757629233346No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCT8600.2662530456562054No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCT8510.2634666765737567No Hit
TGCTTATAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGTG7900.24458128612604915No Hit
TGCTTATAGAACAGGCTCCTCTAGAGACAGTCTCTCGCCAGGGGTATCTA7760.24024693422001786No Hit
TGCTTATAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTGT7720.2390085479611518No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT7580.23467419605512058No Hit
TGCTTATAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCCAG6850.21207364683081478No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTGTTGC6790.21021606744251564No Hit
TGCTTATAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTAT6400.19814180141857146No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG6360.1969034151597054No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGT5760.1783276212767143No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCCT5600.17337407624125No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCCT5190.1606806170878728No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTGTTG4980.15417908922882592No Hit
TGCTTATAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTGTT4910.1520119132758103No Hit
TGCTTATAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGGGG4510.13962805068714956No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT4480.13869926099300003No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCCT4410.1365320850399844No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT4140.12817297779263842No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCT4080.1263153984043393No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCT3980.12321943275717412No Hit
TGCTTATAGAACAGGCTCCTCTTAGCGTCAGGGGTATCTAATCCCAGTGT3920.12136185336887502No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCC3800.1176466945922768No Hit
TGCTTATAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTGT3750.1160987117686942No Hit
TGCTTATAGAACAGGCTCCTCTCGTGGGGTATCTAATCCCAGTGTGTTGC3610.11176435986266296No Hit
TGCTTATAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT3580.11083557016851342No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCT3560.11021637703908037No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCT3430.10619162169776564No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTT3320.10278605948588396No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT3250.10061888353286833No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGGTATCTAATCCCAGTGTGTT3240.10030928696815179No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATTG157.235554E-6295.03
CCATCGC108.462214E-4295.0295
GCTTAGA550.0295.02
TGCTTAT320800.0294.448271
CTTATAG317100.0294.395323
GCTTATA319850.0293.616552
TTATAGA317750.0293.60744
GAACAGG314800.0291.392159
TATAGAA323400.0287.155245
ATAGAAC324500.0284.318186
TAGAACA325550.0282.44977
AGAACAG328000.0280.070138
GCTTAAG450.0262.222232
TGCTTAG850.0260.294131
CTTATGG301.5788828E-9245.833343
GAACCGG650.0226.923089
GCTTATG1700.0216.911762
GCTTTAG850.0208.235292
TGCTTTT650.0204.230771
GAGGCTC150.002848752196.666679
Produced by FastQC (version 0.11.5)