FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-216-6-1-2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-216-6-1-2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252110
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC9634238.21427154813375No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGC178707.088175796279401No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC173736.891039625560272No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC124904.954186664551187No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC84013.332275593986752No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC74062.9376066002935226No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC40431.6036650668359047No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC32681.296259569235651No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTT27971.1094363571456904No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC25281.0027369005592797No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC24650.9777478084962913No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG24350.9658482408472492No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTG20820.8258299948435207No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGT19650.7794216810122566No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT17670.7008845345285788No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTC17310.6866050533497283No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC15930.6318670421641347No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC13340.5291341081274047No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC12810.5081115386140971No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTGT12470.4946253619451827No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTG10180.40379199555749473No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGT10020.3974455594780056No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGT9960.3950656459481972No Hit
TTCTCAGCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTG9570.3795962080044425No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCC9280.3680932926103685No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTC8410.33358454642814644No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTG8150.32327158779897663No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGT7870.3121653246598707No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC7740.30700884534528583No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC7630.302645670540637No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTA6950.2756733172028083No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTC6850.27170679465312764No Hit
TTCTCAGCTAGAACAGGCTCCTCTCGTGGGGTATCTAATCCCAGTGTGTT6790.26932688112331915No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG6450.2558407044544048No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTGT6220.24671770259013923No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAG6090.24156122327555432No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTC5790.22966165562651225No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGT5740.2276783943516719No Hit
TTCTCAGCTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGT5410.2145888699377256No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA5030.19951608424893896No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTC4970.19713617071913056No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCC4740.18801316885486494No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTC4730.18761651659989687No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTC4710.18682321208996072No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTC4510.17889016699059934No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGT4380.17373368767601444No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC4290.17016381738130182No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGACGACGACGACGAGACGGGGT4200.16659394708658917No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA4120.16342072904684463No Hit
TTCTCAGCTAGAACAGGCTCCTCTCGGGGGTATCTAATCCCAGTGTGTTG4100.16262742453690848No Hit
TTCTCAGCTAGAACAGGCTCCTCTTAGCGTCAGGGGTATCTAATCCCAGT4020.15945420649716394No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTC3720.14755463884812184No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTC3450.13684502796398398No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTGTT3400.13486176668914363No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTC3180.1261354170798461No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC3130.12415215580500576No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC2860.11344254492086789No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGACAGTCTCTCGCCAGGGGTATC2850.1130458926658998No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTC2720.10788941335131491No Hit
TTCTCAGCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC2560.10154297727182579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGCT255.293259E-10295.03
CTCAGCT248250.0294.762333
TCTCAGC249150.0294.34882
TCAGCTA248300.0294.049534
CAGCTAG247000.0293.745975
TTCTCAG251450.0293.24021
AGCTAGA247850.0292.679056
GCTAGAA247600.0292.378857
CTAGAAC247800.0290.952368
TAGAACA256450.0280.563489
TTCTCGC700.0231.78571
TTCTCAC550.0214.545441
CTGCTAG150.002848383196.666665
CTCGCTA850.0190.882373
TCGGCTA408.816642E-9184.3754
TCTCGCT1000.0162.252
GCTAGAC1700.0156.176487
TCGCTAG1050.0154.523824
TCAGCTG900.0147.54
TCTCAGA900.0147.52
Produced by FastQC (version 0.11.5)