FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-215-6-1-2-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-215-6-1-2-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215121
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC8408339.08637464496725No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC141756.589314850711925No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCT129386.014289632346447No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC83173.8661962337475186No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC74273.452475583508816No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC59832.7812254498631No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC40701.8919584791814839No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC35641.6567420196075697No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC30161.4020016641796942No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT24551.1412181981303546No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC22881.063587469377699No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT21280.9892107232673704No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTG19560.9092557211987672No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTG17700.8227927538455101No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGC16650.773983014210607No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTG16640.7735181595474175No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCC15710.730286675870789No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC14140.657304493750029No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTGTT12530.5824628929765109No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT11760.5466690839109153No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC10400.4834488497171359No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGT10290.47833544842205084No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA9900.4602061165576582No Hit
GCGAAGTTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGT8540.396985882363879No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTT6720.31238233366338014No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC6560.3049446590523473No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTGT6420.29843669376769355No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTGTTG6320.293788147135798No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCC6120.2844910538720069No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTT6100.2835613445456278No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG6050.28123707122968006No Hit
GCGAAGTTAGAACAGGCTCCTCTCGTGGGGTATCTAATCCCAGTGTGTTG5800.26961570464994117No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAG5720.26589686734442475No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCC5280.2454432621640844No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTGTT5180.24079471553218887No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTG5120.23800558755305157No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT4890.2273139302996918No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC4740.22034111035184847No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCT4710.21894654636227984No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC4620.21476285439357384No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCCA4500.2091845984352992No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCC4030.18733642926539018No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCC3930.18268788263349464No Hit
GCGAAGTTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTA3900.18129331864392598No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGT3760.17478535335927223No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTG3540.16455855076910206No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC3360.15619116683169007No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCC3300.15340203885255277No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCC3270.15200747486298408No Hit
GCGAAGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT3090.14364009092557212No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCC3040.14131581760962436No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTCC2930.13620241631453928No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTT2920.1357375616513497No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC2750.1278350323771273No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCC2650.12318648574523175No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC2640.1227216310820422No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGACAGTCTCTCGCCAGGGGTATCT2620.1217919217556631No Hit
GCGAAGTTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT2610.12132706709247353No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAGGTCC2520.11714337512376755No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGGTATCTAATCCCAGTGTGT2510.116678520460578No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCC2420.112494828491872No Hit
GCGAAGTTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCTT2420.112494828491872No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG2360.10970570051273469No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT2290.1064517178704078No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGT2200.10226802590170184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTT213000.0294.515262
GAAGTTA212200.0294.443943
GCGAAGT214150.0294.311221
GTTAGAA210000.0294.22746
AAGTTAG211200.0294.022254
AGTTAGA211000.0293.881535
TTAGAAC214800.0286.828467
TAGAACA217350.0282.92048
AGAACAG218650.0281.103369
GCGAATT750.0216.333331
AGTTAGG353.967216E-9210.714285
GAAGTTG950.0201.84213
GCGAAGG457.8216544E-11196.666661
GTTAGAC1050.0196.666666
GTTAAAC900.0180.277776
AAGTTAA1950.0173.974374
GAATTAG850.0173.52943
GTTAGGA503.342211E-8147.56
CGAATTA1000.0147.52
TTGGAAC301.1477896E-4147.499987
Produced by FastQC (version 0.11.5)