FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-214-6-1-1-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-214-6-1-1-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196731
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT8171841.53793758990703No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT165198.396744793652246No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT81894.162536661736076No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTGTC78413.985645373632015No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT78253.9775124408456217No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT57932.944629976973634No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCTT43332.2024998602152177No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT35961.8278766437419622No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT26871.3658243998149757No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT24271.2336642420360797No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT19010.9662940766833901No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT17430.8859813654177531No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCCTG15860.806176962451266No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT15840.8051603458529667No Hit
ATCTGTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCTT14700.7472131997499123No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT13960.7095983856128419No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGTA12850.6531761644072362No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG10510.5342320224062298No Hit
ATCTGTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTACC9190.4671353269184826No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCTGT8890.4518860779439946No Hit
ATCTGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACCCT8440.4290122044822626No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT7770.39495554843923936No Hit
ATCTGTAGAACAGGCTCCTCTAGATTGAGACTGTCTCTTATAGGGGTATC7110.36140720069536575No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTACCCT6810.3461579517208777No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCTT6550.33294193594298815No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT6280.31921761186594894No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT5910.3004102047974137No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTACCC5650.2871941890195241No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCTT5380.27346986494248493No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCCTT5220.2653369321560913No Hit
ATCTGTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTGTA4820.2450046001901073No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTTT4730.2404298254977609No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCTT4650.23636335910456413No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCCTT4640.23585505080541447No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT4330.2200974935317769No Hit
ATCTGTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGTAC4250.21603102713858008No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTACCCTG4030.2048482445572889No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCCTT3870.1967153117708953No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCTT3860.1962070034717457No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTCCTT3800.19315715367684808No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACCCT3530.17943282959980889No Hit
ATCTGTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCCAGT3460.17587467150576166No Hit
ATCTGTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTATT3350.17028328021511607No Hit
ATCTGTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTACCC3310.16825004701851767No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGTT3200.16265865572787208No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT3080.15655895613807688No Hit
ATCTGTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCTTTG2970.15096756484743126No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCCTT2950.14995094824913205No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCTT2950.14995094824913205No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTACCCT2930.14893433165083286No Hit
ATCTGTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTGTA2840.14435955695848646No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCCTT2830.14385124865933685No Hit
ATCTGTAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGGGGT2800.14232632376188806No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACCCT2560.13012692458229766No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCCTT2540.12911030798399845No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATACGAAGCACCGCCAGGTCCTT2440.12402722499250246No Hit
ATCTGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT2350.11945245030015605No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT2290.11640260050525846No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTG2280.11589429220610886No Hit
ATCTGTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGTGT2180.11081120921461285No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCTT2010.10216996812906964No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCTT2000.10166165982992005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCT350.0295.09
TCTGTAG195150.0294.16862
ATCTGTA196050.0293.79621
CTGTAGA195400.0293.490263
AACAGGC193000.0292.707289
GAACAGG193500.0292.408268
AGAACAG201000.0281.86447
GTAGAAC202250.0280.92465
TAGAACA202100.0280.18436
TGTAGAA204650.0278.42294
AACCGGC301.5770638E-9245.833319
GAACGGC450.0229.444448
AAAGGCT403.45608E-11221.249989
TCTGAGA600.0196.666662
GAACGGG150.0028479092196.666668
AGAACGG700.0189.642857
GAACCGG255.5495402E-5177.08
GAAAGGC553.1286618E-10160.909098
GCAACGG200.006733482147.49998295
CAATCGG200.006733482147.49998295
Produced by FastQC (version 0.11.5)