FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-213-6-5-8-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-213-6-5-8-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189034
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC7300838.62162362326354No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATC185439.809346466773174No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC84304.4595152194843255No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC76694.056942137393273No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC54222.868267084228234No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC39282.0779330702413326No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGT32151.7007522456277706No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTCAA31891.6869981061607964No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC28361.500259212628416No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC22301.1796819619750945No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTC19831.0490176370388395No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAAT18961.0029941703608876No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT18720.9902980416221421No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG17430.9220563496513855No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC14940.7903340139869017No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC14830.7845149549816435No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC14380.7607097135964959No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTCA14220.7522456277706656No Hit
TCTCAATCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTG13400.7088671879132855No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTC11550.611001195552123No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA10850.5739708200641155No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC9650.5104901763703884No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC8580.45388660241014844No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTC7140.377709829977676No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTCAA7040.37241977633653206No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGT6920.3660717119671593No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTCA6680.3533755832284139No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGT6380.3375054223049822No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAG6220.3290413364791519No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCC6120.32375128283800797No Hit
TCTCAATCTAGAACAGGCTCCTCTCGTGGGGTATCTAATCCCAGTGTCAA6050.3200482452892073No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTC5680.30047504681697473No Hit
TCTCAATCTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGT5580.2951849931758308No Hit
TCTCAATCTAGAACAGGCTCCTCTCGGGGGTATCTAATCCCAGTGTCAAT5020.2655606927854248No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGTATCTAATCCCAGTGTCAAT4990.2639736766930817No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCC4970.2629156659648529No Hit
TCTCAATCTAGAACAGGCTCCTCTTAGCGTCAGGGGTATCTAATCCCAGT4940.2613286498725097No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC4900.25921262841605214No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTC4850.2565676015954802No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTC4620.24440047822084915No Hit
TCTCAATCTAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGG4550.24069744067204843No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGACGAGACGGGTATCTAATCCCA4410.23329136557444694No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTC4080.21583418855867198No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTC3810.2015510437275834No Hit
TCTCAATCTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCT3790.20049303299935461No Hit
TCTCAATCTAGAACAGGCTCCTCTACGAGACGGGGTATCTAATCCCAGTG3780.19996402763524024No Hit
TCTCAATCTAGAACAGGCTCCTCGTCGGGGTATCTAATCCCAGTGTCAAT3700.19573198472232506No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC3690.1952029793582107No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTC3650.19308695790175312No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGACAGTCTCTCGCCAGGGGTATC3380.17880381307066454No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTC3380.17880381307066454No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC3260.17245574870129182No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGACGACGACGACGAGACGGGGT3260.17245574870129182No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGAGGGTATCTAATCCCAGTGTC3240.17139773797306304No Hit
TCTCAATCTAGAACAGGCTCCTCTGTCTCTGGGGTATCTAATCCCAGTGT3070.1624046467831184No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTC3000.15870160923431764No Hit
TCTCAATCTAGAACAGGCTCCTCTGGGGTATCTAATCCCAGTGTCAATCA2880.15235354486494493No Hit
TCTCAATCTAGAACAGGCTCCTCTACACATCTGACGCTGCCGACGAGGGT2700.14283144831088587No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTC2610.13807040003385634No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATTGAGACTGTCTCTTATAGGGGT2500.13225134102859804No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTGGGGTATCTAATCCCAGTGTC2440.12907730884391166No Hit
TCTCAATCTAGAACAGGCTCCTCTACACATCTGACGCTGCCGACGAGGGG2320.12272924447453896No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACCACGAGACGGGGTATCTAATCC2320.12272924447453896No Hit
TCTCAATCTAGAACAGGCTCCTCGGGGTATCTAATCCCAGTGTCAATCAC2280.1206132230180814No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTC2170.1147941640128231No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGAGACGGGTATCTAATCCCAGT2120.11214913719225113No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGAGGGGTATCT2110.11162013182813675No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTC2070.10950411037167916No Hit
TCTCAATCTAGAACAGGCTCCTCCCACGAGGGGGTATCTAATCCCAGTGT2060.10897510500756477No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTC2040.10791709427933599No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC2030.1073880889152216No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC1940.10262704063819207No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGTATCTAATCCCAGTGTCAATCA1940.10262704063819207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCT186500.0293.971863
CTCAATC187450.0293.504942
AATCTAG185500.0293.250675
CAATCTA187250.0292.085454
TCTCAAT189200.0291.49181
CTAGAAC186350.0290.25098
TCTAGAA186900.0289.870247
ATCTAGA187700.0289.656376
TAGAACA192450.0280.66779
CTCAATT457.8216544E-11196.666662
CTCATCT1050.0154.523822
TCTGAAC709.094947E-12147.57
TCTCAAC709.094947E-12147.51
TCTCATC1350.0120.185181
TCATCTA1500.0108.1666643
CTCAACC601.4661897E-598.333332
CCAGCCA455.7625934E-498.33333295
CAATCTG1259.094947E-1294.44
CATCTAG1750.092.714284
TCAACCT508.7593676E-488.53
Produced by FastQC (version 0.11.5)