FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-212-6-1-1-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-212-6-1-1-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185931
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT7683941.326621165916386No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT148367.9793041504644195No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT86494.651725640156832No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTGT84434.5409318510630285No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT69163.7196594435570183No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT52442.820401116543234No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT35851.9281346305887668No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT21991.1826968068799717No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT21241.142359262306985No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT20211.086962367760083No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCT17590.9460498787184493No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT17490.9406715394420512No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT14950.8040617218215359No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCTG14340.7712538522355067No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG13720.7379081487218376No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCCT10970.5900038186208862No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT10920.5873146489826871No Hit
TGCTTATAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTAC9310.5007233866326756No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT8920.4797478634547225No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT8090.4351076474606171No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTACCC7500.4033754457298675No Hit
TGCTTATAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACCC7220.3883160957559525No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT6770.3641135690121604No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTACC6600.35497039224228344No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGT6380.3431380458342073No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTT6140.33023003157085157No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACCC6110.3286165297879321No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA5840.3140950137416569No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT5760.30979234232053826No Hit
TGCTTATAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGTA5720.30764100660997895No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCT5460.2936573244913436No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT5050.2716061334581108No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCCT4990.26837912989227186No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG4260.22911725317456477No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCCT4260.22911725317456477No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCT4190.22535241568108602No Hit
TGCTTATAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGGGG4050.21782274069412846No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTACCCT3870.20814172999661165No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCT3860.20760389606897184No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCT3780.20330122464785322No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCT3650.19630938358853553No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCCT3570.19200671216741694No Hit
TGCTTATAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTAT3560.19146887823977712No Hit
TGCTTATAGAACAGGCTCCTCTTAGCGTCAGGGGTATCTAATCCCAGTGT3450.18555270503573906No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTACCC3170.170493355061824No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCT2990.1608123443643072No Hit
TGCTTATAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCCAG2870.15435833723262932No Hit
TGCTTATAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCTTT2630.14145032296927354No Hit
TGCTTATAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTGT2610.1403746551139939No Hit
TGCTTATAGAACAGGCTCCTCTCGTGGGGTATCTAATCCCAGTGTACCCT2590.13929898725871426No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCC2570.13822331940343463No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT2440.13123147834411691No Hit
TGCTTATAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGTG2160.11617212837020185No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACCC2140.1150964605149222No Hit
TGCTTATAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT2120.11402079265964256No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT2110.11348295873200274No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCC2100.11294512480436292No Hit
TGCTTATAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTGT2050.1102559551661638No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCT2050.1102559551661638No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTCCT2030.10918028731088415No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAGGTCCT1970.1059532837450452No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT1950.10487761588976556No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT1930.10380194803448592No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCT1930.10380194803448592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTAT184750.0294.840331
CTTATAG183850.0294.277953
TTATAGA183700.0294.116764
GCTTATA185050.0293.96382
GAACAGG181800.0292.32269
TATAGAA187600.0286.901645
ATAGAAC188300.0284.11186
TAGAACA189000.0282.357157
AGAACAG189800.0280.623028
TGCTTTA450.0262.222231
CTTTAGA400.0258.1253
GACCAGG301.5770638E-9245.833349
GCTTAAG301.5770638E-9245.833342
GCTTTAG450.0229.444442
TGCTTAG202.2790544E-5221.251
GCTTATG202.2790544E-5221.252
CTTAAGA353.963578E-9210.71433
ACTTCGA150.0028477837196.66667295
GAACGGC304.659796E-7196.666679
TAGGCTC150.0028477837196.666677
Produced by FastQC (version 0.11.5)