FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-211-6-4-6-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-211-6-4-6-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178254
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC7250340.67398207052857No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC148848.349882751579207No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC88344.95584951810338No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCC57473.2240510731876983No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG54213.0411659766400754No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC53152.9817002704006645No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC45202.5357074736050804No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGATCTAAAGCACCGCCAAGTC29301.6437218800139128No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC28921.6224039853243124No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTC27811.5601332929415328No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC26081.4630807723809844No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTG19291.0821636541115487No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC17050.9565002748886421No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC15620.8762776711883044No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC10270.57614415384788No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC9980.559875234216343No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC9560.536313350612048No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT8980.5037755113489739No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC7990.4482367857102786No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGC7870.44150481896619426No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC7790.4370168411368048No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTC7270.4078449852457729No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTC6420.3601602208085092No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTC6410.35959922357983554No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGCC5700.3197684203440035No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC5580.3130364535999192No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTTG5190.2911575616816453No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC4920.27601063650745566No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGT4810.2698396669920451No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTC4140.2322528526709078No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTC3990.22383789424080244No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGATCTAAAGCACCGCCAAGTC3800.21317894689600234No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC3780.21205695243865494No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCGAGTC3760.21093495798130754No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGAATATGAAGCACCGCCAGGTC3710.2081299718379391No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC3670.20588598292324437No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCTGGTC3650.20476398846589697No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGT3530.1980320217218127No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG3440.19298304666374946No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTG3420.1918610522064021No Hit
TTCTCAGCTAGAACAGGCTCCTCTCCTTACCTTATCTGCTGCTACCCAAT3310.1856900826909915No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGG3180.17839711871823352No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCGAGTC3160.17727512426088615No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTTGTG2990.16773817137343341No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC2940.16493318523006498No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTC2910.16325019354404388No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG2910.16325019354404388No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTT2870.16100620462934914No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG2830.15876221571465438No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGG2800.1570792240286333No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTG2760.15483523511393854No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTTG2700.1514692517418964No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCC2540.14249329608311737No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTGTG2450.13744432102505413No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACCTTACATGTAAAAGGGGTATC2420.13576132933903307No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGCTTCTCTGCAGATCTTTTGTG2370.13295634319566463No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTG2360.13239534596699093No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT2360.13239534596699093No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAAGAATAAAGAACCGCCAAGTCT2290.1284683653662751No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT2290.1284683653662751No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA2280.1279073681376014No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTC2270.12734637090892773No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAAT2260.12678537368025403No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTTGTG2220.12454138476555926No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAGGTC2210.12398038753688558No Hit
TTCTCAGCTAGAACAGGCTCCTCTGGGGGGGATGGAAACACCATACCACC2110.11837041525014867No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGTCGGGGGTATCTAATCCCAGT2110.11837041525014867No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAATCTCTAGTAATTAGGGAGGGG2050.11500443187810652No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC2020.11332144019208544No Hit
TTCTCAGCTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCT1960.1099554568200433No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGAATATAAAGCACTGCCAAGTC1930.10827246513402224No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTGTAAAGCACCGCCAAGTC1870.1049064817619801No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTT1810.10154049838993796No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCGCCGCCAAGTC1790.10041850393259057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAG305.456968E-12294.999974
CTCAGCT175850.0294.748353
TCTCAGC176450.0294.665622
TTCTCAG177000.0294.583341
TCAGCTA175350.0294.49534
CAGCTAG174900.0293.903665
GCTAGAA174700.0293.05817
AGCTAGA175800.0292.147346
CTAGAAC175200.0291.548258
TAGAACA179750.0283.593879
CTGCTAG202.2788974E-5221.255
TTCTCGC403.45608E-11221.251
TCGGCTA150.002847686196.666664
CTCGGCT150.002847686196.666663
TCAGCTG1000.0177.04
GCTAGAC1350.0174.81487
GCTGAAC850.0173.52947
TTCTCAC603.6379788E-12172.083331
TCTCGCT605.7480065E-10147.499982
CTCGCTA605.7480065E-10147.499983
Produced by FastQC (version 0.11.5)