FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-210-6-4-6-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-210-6-4-6-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173310
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC7341342.35935606716289No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC1753110.115400150020196No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC77184.4532917892793265No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC55933.227165195314754No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC38942.246840920893197No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCT38372.2139518781374417No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT38242.206450868386129No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC23751.370376781489816No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC22691.3092147019791125No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC19571.1291904679476084No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT16120.9301252091627719No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC15890.9168541919104495No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT13090.7552939818821764No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC13030.7518319773815706No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCC12920.7454849691304599No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC12620.7281749466274307No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA12260.7074029196237955No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGG11760.6785528821187468No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC11420.6589348566153136No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCC9730.5614217298482488No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT9550.5510357163464313No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC8620.49737464658704056No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTCC8450.4875656338353239No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCC7950.45871559633027525No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT7010.40447752582078356No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTT5510.3179274133056373No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA5500.3173504125555363No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCC5270.3040793953032139No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCGAGTCC5110.2948473833015983No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTTGT5030.29023137730079046No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCC5020.2896543765506895No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGG4930.2844613697997807No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCC4310.24868732329352028No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCC4260.2458023195430154No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGC3800.21926028503837053No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGGC3540.20425826553574522No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCC3530.20368126478564424No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTG3320.19156424903352373No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGGTCTGAAGCACCGCCAAGTC3210.18521724078241303No Hit
CCACGTCTAGAACAGGCTCCTCTGGAGGGATATGAAGCACCGCCAGGTCC3160.18233223703190815No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGGTATAAAGCACCGCCAAGTCC3130.1806012347816052No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG3090.17829323178120132No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAG3000.17310022503029254No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCC2930.16906121977958571No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGTATGAAGCACCGCCAGGTCC2820.16271421152847498No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTTC2770.1598292077779701No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTG2710.15636720327736425No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC2710.15636720327736425No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATACGAAGCACCGCCAGGTCC2640.15232819802665742No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAGGTCC2580.14886619352605157No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTTGTG2530.1459811897755467No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA2530.1459811897755467No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGT2490.14367318677514282No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG2360.13617217702383014No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC2340.13501817552362816No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGTACCGCCAAGTCC2260.13040216952282038No Hit
CCACGTCTAGAACAGGCTCCTCTTATACACATCTCCGAGGGGTATCTAAT2200.12694016502221453No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTG2130.1229011597715077No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTT1950.11251514626969014No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGATGTAAAGCACCGCCAAGTCC1940.11193814551958918No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCT1930.1113611447694882No Hit
CCACGTCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1890.10905314176908429No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCGTACCACCA1790.10328313426807455No Hit
CCACGTCTAGAACAGGCTCCTCTACACATCTGACGCTGCCGACGAGGGGG1780.10270613351797356No Hit
CCACGTCTAGAACAGGCTCCTCTTATACACATCTGACGCTGCCGAAGAGG1770.10212913276787261No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTT1740.10039813051756967No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCAG108.4588357E-4295.04
CCACGGC108.4588357E-4295.01
CGTCTAG169600.0294.826054
GTCTAGA169700.0294.478525
CCACGTC172200.0294.400421
CACGTCT171150.0294.396732
ACGTCTA170500.0294.39443
CTAGAAC170350.0292.229257
TCTAGAA171800.0289.93456
TAGAACA174750.0284.027168
AGAACAG177650.0279.473669
CCACGTT650.0226.923081
ACGTTCT202.2787883E-5221.253
CGTTAGA500.0206.54
CGTCAGA1300.0181.538474
CGTCTAA900.0180.277774
TTCTAGA255.548406E-5177.06
CACGTTC255.548406E-5177.02
CGCTAGA351.0041349E-6168.571434
CCACGCT351.0041349E-6168.571431
Produced by FastQC (version 0.11.5)