FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-21-4-3-8-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-21-4-3-8-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12119
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT202016.66804191764997No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC185115.273537420579256No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATCA9307.673900486838849No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCAA4493.7049261490221967No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC4243.4986385015265284No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT3022.491954781747669No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTCA2662.194900569353907No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTCAA2582.128888522155293No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT2492.054624969056853No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG2452.021618945457546No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAATC2221.8318343097615315No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG2211.8235828038617046No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGT2131.7575707566630911No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTCAAT1981.6337981681656903No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC1461.2047198613747008No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTCA1451.1964683554748743No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC1301.0726957669774735No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1301.0726957669774735No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTCA1291.0644442610776466No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG1210.9984322138790328No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTCA900.7426355309844046No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC840.6931264955854444No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTG830.6848749896856177No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTCA810.6683719778859643No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTCAA780.643617460186484No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT750.6188629424870039No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTGTCA740.6106114365871771No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG700.5776054129878703No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTGT700.5776054129878703No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC580.47858734218994964No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTC560.4620843303902962No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCCGTGGGGTATCTAATCCCAGT510.4208268008911626No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA490.4043237890915092No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG490.4043237890915092No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTG480.39607228319168253No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC480.39607228319168253No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGATCAGGGGTATCTAATCCCAGT450.3713177654922023No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCA410.3383117418928955No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTG390.321808730093242No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGTGGGGGTATCTAATCCCAGTGTC390.321808730093242No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGTC380.3135572241934153No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA350.28880270649393514No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTGCAGCAGGGGTATCTAATCCCAG340.28055120059410843No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTG310.25579668289462826No Hit
GCGAAGTTAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGTG310.25579668289462826No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTC300.24754517699480155No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTCAA290.23929367109497482No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTC280.2310421651951481No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTCAA280.2310421651951481No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTCTCGTGGGGTATCTAATCCCAGT280.2310421651951481No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC280.2310421651951481No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTGTC260.21453915339549468No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAGT250.20628764749566794No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGT240.19803614159584126No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTGTCA230.1897846356960145No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA230.1897846356960145No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTTGGGGGTATCTAATCCCAGTGT230.1897846356960145No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTTCGGGGTATCTAATCCCAGTGT220.18153312979618783No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTCAA220.18153312979618783No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC210.1732816238963611No Hit
GCGAAGTTAGAACAGGCTCCTCTGGACATCGGGGTATCTAATCCCAGTGT210.1732816238963611No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGT200.16503011799653436No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTTGGGGTATCTAATCCCAGTGTC200.16503011799653436No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC190.15677861209670765No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGTCGGGGGTATCTAATCCCAG190.15677861209670765No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTTGGCGGGGGTATCTAATCCCAG180.14852710619688092No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTATTCGGGGTATCTAATCCCAG170.14027560029705421No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTGT150.12377258849740078No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA150.12377258849740078No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT150.12377258849740078No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTGT150.12377258849740078No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTCAA150.12377258849740078No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC140.11552108259757406No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTGTC140.11552108259757406No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTTCTCGGGGGTATCTAATCCCA140.11552108259757406No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAG140.11552108259757406No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGGGGGTATCTAATCCCAGTGTC130.10726957669774734No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTGT130.10726957669774734No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTCGTCGGGGTATCTAATCCCAGT130.10726957669774734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTT12000.0295.02
GCGAAGT12050.0295.01
GTTAGAA11750.0295.06
AGTTAGA11750.0295.05
GAAGTTA12000.0293.770843
AAGTTAG11850.0293.755284
TTAGAAC11800.0293.757
TAGAACA12700.0272.933048
AGAACAG12950.0267.66419
AGTTAAA202.2269402E-5221.249985
AAGTTAA301.1213938E-4147.499984
GTTAAAC200.0066560986147.499986
GGCGAGC1550.059.00000480-84
AGCACCG1850.059.00000435-39
TTAACTG1550.059.000004100-104
CTACAGG201.8334501E-459.025-29
GTTGATT1600.059.095-99
ACCGGGG550.059.030-34
TTGTTGA1750.059.090-94
GGGTCTG257.745257E-659.025-29
Produced by FastQC (version 0.11.5)