FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-209-6-5-7-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-209-6-5-7-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172794
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT5048329.2157135085709No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCTG2643215.296827436137828No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT107706.232855307475954No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT46132.6696528814657916No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAGCT39472.28422283181129No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTCA35852.074724816833918No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCATCCAGC25821.4942648471590447No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT24801.4352350197344816No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTCATCCAGC22521.303285993726634No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT21811.2621966040487516No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT18131.0492262462816995No Hit
CTAGGTAGAACAGGCTCCTCTCTCGGGGTATCTAATCCCAGTCATCCAGC17431.0087155804020973No Hit
CTAGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCAT15140.8761878305959697No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCTT15140.8761878305959697No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCATC14920.8634559070338091No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCAT12560.7268770906397213No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT12130.7019919673136799No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTCTGAGACACCGTCAGGTCCTT11140.6446983112839566No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT11110.6429621398891164No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT10430.6036089216060743No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT10310.5966642360267139No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTC9830.5688854937092723No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCTT9070.5249024850399898No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGTT8790.5086982186881489No Hit
CTAGGTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCTT8760.5069620472933088No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT8750.5063833234950288No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTCA8250.4774471335810271No Hit
CTAGGTAGAACAGGCTCCTCTATACATAGGGGGTATCTAATCCCAGTCAT8150.4716598955982268No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATCCC7710.44619604847390537No Hit
CTAGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT7500.4340428487100247No Hit
CTAGGTAGAACAGGCTCCTCTAGACACGAGACGGGGTATCTAATCCCAGT7400.4282556107272243No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGAAAATTGGGGGGTATCTAATCCCA7390.4276768869289443No Hit
CTAGGTAGAACAGGCTCCTCTATACATCGGGGTATCTAATCCCAGTCATC7160.41436623956850355No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCGTGGTCTCGGGGTATCTAATCCC7040.4074215539891431No Hit
CTAGGTAGAACAGGCTCCTCTATATCCGGGGGTATCTAATCCCAGTCATC6920.40047686840978275No Hit
CTAGGTAGAACAGGCTCCTCTAGTGCCGACGGGGGTATCTAATCCCAGTC6860.3970045256201026No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCATCC6600.3819577068648217No Hit
CTAGGTAGAACAGGCTCCTCTGTCTCGGGGTATCTAATCCCAGTCATCCA6310.36517471671470075No Hit
CTAGGTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCCAGT6270.3628598215215806No Hit
CTAGGTAGAACAGGCTCCTCTATACGGGGTATCTAATCCCAGTCATCCAG6230.36054492632846047No Hit
CTAGGTAGAACAGGCTCCTCTAGTGAGTCGTATTAAGGGGTATCTAATCC6170.35707258353878024No Hit
CTAGGTAGAACAGGCTCCTCGTCTCGTGGGGTATCTAATCCCAGTCATCC6050.3501278979594199No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCTT5880.34028959338865933No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTCATCCAG5460.3159831938608979No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTCATC5190.30035765130733705No Hit
CTAGGTAGAACAGGCTCCTCTAGCCGACGAAGGGGTATCTAATCCCAGTC5160.298621479912497No Hit
CTAGGTAGAACAGGCTCCTCGTCTCGTGGTCTCGGGGTATCTAATCCCAG4660.26968528999849534No Hit
CTAGGTAGAACAGGCTCCTCTTATACACATCTGACGGCGAGAGGGGTATC4650.2691065662002153No Hit
CTAGGTAGAACAGGCTCCTCTATACACATCTGACGCTGCCACGAGGGTAT4490.25984698542773477No Hit
CTAGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCAG4460.25811081403289465No Hit
CTAGGTAGAACAGGCTCCTCTGAGGGGGTATCTAATCCCAGTCATCCAGC4150.24017037628621365No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAGGTCCTT3890.22512355753093277No Hit
CTAGGTAGAACAGGCTCCTTAGGGGTATCTAATCCCAGTCATCCAGCTGT3650.211234186372212No Hit
CTAGGTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTCATCC3600.20834056738081183No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAAGTCCTT3560.20602567218769172No Hit
CTAGGTAGAACAGGCTCCTCTATACACAGGGGTATCTAATCCCAGTCATC3460.20023843420489137No Hit
CTAGGTAGAACAGGCTCCTCTAGCACGGGGGTATCTAATCCCAGTCATCC3430.1985022628100513No Hit
CTAGGTAGAACAGGCTCCTCTAGCCCACAGACGGGGTATCTAATCCCAGT3270.18924268203757075No Hit
CTAGGTAGAACAGGCTCCTCTAGGGTATCTAATCCCAGTCATCCAGCTGT3260.18866395823929072No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCTTT3190.1846128916513305No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT3130.1811405488616503No Hit
CTAGGTAGAACAGGCTCCTCTAGAGACACGAGACGGGTATCTAATCCCAG3130.1811405488616503No Hit
CTAGGTAGAACAGGCTCCTCTAGCCCACGAGAGGGGTATCTAATCCCAGT3120.18056182506337026No Hit
CTAGGTAGAACAGGCTCCTCTAGACGAGGGTATCTAATCCCAGTCATCCA2770.1603064921235691No Hit
CTAGGTAGAACAGGCTCCTCTAGCTTCTACGGGGGTATCTAATCCCAGTC2720.15741287313216892No Hit
CTAGGTAGAACAGGCTCCTCTAGACGAGACGACGACGACGAGGGTATCTA2660.15394053034248875No Hit
CTAGGTAGAACAGGCTCCTCTAGGTATCTAATCCCAGTCATCCAGCTGTC2650.15336180654420872No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGGGTATCTAATCCCAGTCATCCA2520.14583839716656827No Hit
CTAGGTAGAACAGGCTCCTCTAGCTTCTCTCGGGGTATCTAATCCCAGTC2520.14583839716656827No Hit
CTAGGTAGAACAGGCTCCTCGGGGTATCTAATCCCAGTCATCCAGCTGTC2490.14410222577172818No Hit
CTAGGTAGAACAGGCTCCTCTAGCTGTCGGGGTATCTAATCCCAGTCATC2480.14352350197344815No Hit
CTAGGTAGAACAGGCTCCTCGAGACGGGGTATCTAATCCCAGTCATCCAG2170.12558306422676715No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGTATCTAATCCCAGTCATCCAGCT1990.11516603585772654No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGTATCTAATCACAGTCATCCAGCTG1940.11227241686632637No Hit
CTAGGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1870.10822135027836616No Hit
CTAGGTAGAACAGGCTCCTGTCTCGGGGTATCTAATCCCAGTCATCCAGC1780.10301283609384586No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCAGTCATCCAGCTGT1760.10185538849728577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGAA169800.0293.43644
TAGGTAG170900.0293.273832
CTAGGTA171500.0293.193881
AGGTAGA170850.0293.014343
AACAGGC169850.0290.831639
GAACAGG170600.0289.898868
GTAGAAC173900.0285.415475
TAGAACA177300.0279.443026
AGAACAG177800.0278.325387
AGGTTAG950.0248.421073
TAGGTTA1000.0236.02
CTAGGTT1250.0224.21
GAAAGGC403.45608E-11221.258
CTAGGAG700.0168.571431
GTTAGAA1350.0163.88895
GGTTAGA1450.0162.758624
AACCGGC301.1474011E-4147.499989
GACAGGC1250.0141.599998
GGTAGAC1600.0138.281254
GGTCGAA453.508021E-6131.111114
Produced by FastQC (version 0.11.5)