FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-208-6-5-7-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-208-6-5-7-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171682
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC7235842.14652671800189No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC119586.965203108071901No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAG96675.630759194324391No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC56933.3160144919094607No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCA47452.763830803462215No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC42202.4580328747335187No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG26341.534231893850258No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC23991.3973509162288418No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC23561.3723046096853484No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCAT21141.2313463263475495No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC20531.195815519390501No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCGAGTC18031.050197458091122No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCTGGGGTATCTAATCCCAGTCAT16630.9686513437634696No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT15580.9074917580177304No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA15200.8853578127002248No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC15160.8830279237194347No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATC14740.858564089421139No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTCA14000.8154611432765229No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCGCGGGGGTATCTAATCCCAGT13020.7583788632471663No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC12310.7170233338381427No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC11860.6908120828042543No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTAGGGGGTATCTAATCCCAGTCA11440.6663482485059588No Hit
TCTCAATCTAGAACAGGCTCCTCTCGGGGTATCTAATCCCAGTCATCCAG11030.6424668864528605No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG9780.569657855803171No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC9730.5667454945771834No Hit
TCTCAATCTAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGG9650.5620857166156032No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTC9570.5574259386540231No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC9490.552766160692443No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC9060.5277198541489497No Hit
TCTCAATCTAGAACAGGCTCCTCTATGTCTCGTGGGGTATCTAATCCCAG8150.4747148798359758No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGTCGCGTCAGGGGTATCTAATC6620.3855966263207558No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATTGAGGGGGTATCTAATCCCAGT5870.3419112079309421No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG5200.3028855675027085No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTC4430.25803520462249974No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGCAGGGGTATCTAATCCCAGTC3920.2283291201174264No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACCACGAGACGGGGTATCTAATCC3830.22308686991064874No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATTGAGGGGTATCTAATCCCAGTC3700.21551473072308103No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGACCACGAGACTGGGGTATCTAA3690.2149322584778835No Hit
TCTCAATCTAGAACAGGCTCCTCTTATACACATCTGACGCTGCCGACGAG3530.20561270255472328No Hit
TCTCAATCTAGAACAGGCTCCTCTACAGTAAGCTTTTTCCCGCTAGCTTC3260.18988595193439034No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA3090.17998392376603256No Hit
TCTCAATCTAGAACAGGCTCCTCTGACGCTGCCGACGAGGTATCTAATCC2680.15610256171293438No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC2560.1491128947705642No Hit
TCTCAATCTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCT2410.14037581109260144No Hit
TCTCATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC2290.13338614415023123No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGAGCCTGAGGGGTATCTAATCC2260.1316387274146387No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCCCACGAGAGGGTATCTAATCCCA2020.1176593935298983No Hit
TCTCAATCTAGAACAGGCTCCTCTGGGGTATCTAATCCCAGTCATCCAGC2010.11707692128470079No Hit
TCTCAATCTAGAACAGGCTCCTCTGGAGGGATGTGAAGCACCGCCAGGTC2000.11649444903950328No Hit
TCTCAATCTAGAACAGGCTCCTCGGGGTATCTAATCCCAGTCATCCAGCT1770.1030975873999604No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC1740.10135017066436784No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCT169100.0293.866063
CTCAATC169700.0293.60932
AATCTAG168100.0292.981845
TCTCAAT171350.0291.8151
TCTAGAA168050.0291.66477
CAATCTA169550.0291.346224
ATCTAGA169350.0290.5586
CTAGAAC168700.0290.191168
TAGAACA173500.0281.567729
TCAGAGG202.2787504E-5221.25295
TCTGAAC1050.0196.666677
CTCAGCT551.8189894E-12187.727262
CTCATCT900.0180.277772
TCTCAGC603.6379788E-12172.083341
AATCTGG351.0041076E-6168.571435
TCTCATC1050.0154.523821
ACCGTGA200.0067327386147.5295
TCTCAAG709.094947E-12147.51
CAATCTG2050.0136.70734
CTCAAGC802.910383E-11129.06252
Produced by FastQC (version 0.11.5)