FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-207-6-5-7-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-207-6-5-7-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170050
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT8113947.714789767715374No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT87645.153778300499853No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT83014.881505439576595No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCTG61043.589532490443987No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT58033.4125257277271395No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAGCT29421.730079388415172No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT28591.681270214642752No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG24931.4660394001764185No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT24921.4654513378418113No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCATCC23941.4078212290502794No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT22751.3378418112319905No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCAG19131.124963246104087No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT18871.109673625404293No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTCA17701.040870332255219No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATCCA17381.0220523375477801No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATCCA14820.8715083798882681No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT11750.6909732431637753No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT11630.6839164951484857No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCCAG11050.6498088797412526No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT10920.6421640693913555No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT10270.60394001764187No Hit
ATCTGTAGAACAGGCTCCTCTAGGGTATCTAATCCCAGTCATCCAGCTGT8740.5139664804469274No Hit
ATCTGTAGAACAGGCTCCTCTAGATGCAGGGGTATCTAATCCCAGTCATC8690.5110261687738901No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCTT8620.5069097324316377No Hit
ATCTGTAGAACAGGCTCCTCTAGAGAGGGGTATCTAATCCCAGTCATCCA8510.5004410467509556No Hit
ATCTGTAGAACAGGCTCCTCTACACGAGACGGGGTATCTAATCCCAGTCA8070.47456630402822697No Hit
ATCTGTAGAACAGGCTCCTCTAGAGAGATATGAAGCACCGCCAGGTCCTT7450.438106439282564No Hit
ATCTGTAGAACAGGCTCCTCTAGGAGGGGTATCTAATCCCAGTCATCCAG7450.438106439282564No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTC7020.4128197588944428No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCTT6560.38576889150249927No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT4630.27227286092325786No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT4130.24286974419288443No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGAACCGCCAAGTCCTT3720.21875918847397824No Hit
ATCTGTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCTT3580.21052631578947367No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTACTT3560.20935019112025877No Hit
ATCTGTAGAACAGGCTCCTCGAGAGGGGTATCTAATCCCAGTCATCCAGC2960.17406645104381063No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT2830.16642164069391358No Hit
ATCTGTAGAACAGGCTCCTCTAGAGTGATATGAAGCACCGCCAGGTCCTT2610.15348426933254924No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCTT2510.14760364598647457No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT2180.12819758894442812No Hit
ATCTGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT2050.12055277859453102No Hit
ATCTGTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT2030.11937665392531607No Hit
ATCTGTAGAACAGGCTCCTCTAGAGACCACGAGACGGGGTATCTAATCCC2030.11937665392531607No Hit
ATCTGTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT1960.1152602175830638No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT1780.10467509556012938No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGGC108.458697E-4295.09
GAACCGG450.0295.08
TCTGTAG167300.0294.73552
CTGTAGA167650.0294.120183
ATCTGTA168850.0294.03911
GAACAGG166200.0292.515058
AACAGGC166250.0292.427069
TGTAGAA170450.0287.817544
GTAGAAC169700.0287.525025
TAGAACA172000.0282.822666
AGAACAG172700.0281.67637
AACCGGC450.0262.222239
GACAGGC850.0225.588248
TCTGTAA900.0213.055562
GAAAGGC353.963578E-9210.714288
TCTGTAT750.0177.02
TCTGTGA700.0168.571432
ATCTGTT451.7796992E-8163.888891
AACGGCT200.006732684147.59
CTGTAAA1250.0141.599993
Produced by FastQC (version 0.11.5)