FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-206-6-8-1-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-206-6-8-1-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173616
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC7600543.77764722145424No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC121547.000506865726661No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTG95845.520228550364021No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC72744.189706017878537No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC60773.5002534328633303No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC40502.332734310201825No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC26251.5119574232789605No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC25941.4941019260897612No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC21951.2642843977513594No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT19471.1214404202377661No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC18591.0707538475716525No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC17631.0154594046631649No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT17150.9878121832089208No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCC14760.8501520597179982No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCT14030.8081052437563359No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC13290.7654824440143765No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC10300.5932632937056492No Hit
CGATTCCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCC8930.5143535158049949No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT8760.5045617915399503No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTG8550.49246613215371854No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTA8520.4907381808128283No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTACC7130.4106764353515805No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA6850.3945488895032716No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC6270.3611418302460603No Hit
CGATTCCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACC5420.3121832089208368No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTG4750.2735922956409548No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCC4660.26840844161828403No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTACCC4610.2655285227168003No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTACC4610.2655285227168003No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCC4430.25516081467145885No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACC3960.22808957699751173No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAG3680.21196203114920284No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCC3610.20793014468712562No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTAC3550.2044742420053451No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCT3230.1860427610358492No Hit
CGATTCCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGT3170.18258685835406877No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACC3110.17913095567228826No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCC2920.1681872638466501No Hit
CGATTCCTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTA2890.16645931250575985No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCCA2680.15436365311952815No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT2550.14687586397567046No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT2460.14169200995299971No Hit
CGATTCCTAGAACAGGCTCCTCTACACATCTGACGCTGCCGACGAGGGGG2460.14169200995299971No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCC2430.13996405861210948No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT2420.13938807483181273No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG2370.136508155930329No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTAC2360.13593217215003225No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCC2300.13247626946825178No Hit
CGATTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT2250.12959635056676805No Hit
CGATTCCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT2250.12959635056676805No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTCC2250.12959635056676805No Hit
CGATTCCTAGAACAGGCTCCTCTACGAGACGGGGTATCTAATCCCAGTGT2070.1192286425214266No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC2070.1192286425214266No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTAC2050.11807667496083311No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG2000.11519675605934937No Hit
CGATTCCTAGAACAGGCTCCTCTTAGCGTCAGGGGTATCTAATCCCAGTG1980.11404478849875586No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTG1910.11001290203667864No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAGGTCC1860.10713298313519491No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGT1770.1019491291125242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTAA1050.0295.04
TCCTAGC108.4588496E-4294.999975
GACGGTA108.4588496E-4294.99997295
ATTCCTA171000.0294.913763
TTCCTAG169900.0294.91324
GATTCCT171700.0294.82822
TCCTAGA169850.0294.739475
CCTAGAA168950.0294.65086
CTAGAAC170500.0291.712627
CGATTCC175400.0289.365751
TAGAACA175000.0283.87438
AGAACAG177250.0279.85479
GATTCGT650.0272.30772
ATTCCTG550.0268.181823
TTCCTGA450.0262.222234
ATTCGTA700.0252.857133
TTCGTAG750.0236.000024
CGATTCG750.0236.000021
GATTTCT202.2787955E-5221.249982
TTCCAAG202.2787955E-5221.249984
Produced by FastQC (version 0.11.5)