FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-205-6-5-7-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-205-6-5-7-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169058
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT6868840.629843012457265No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT161589.55766659962853No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCT56913.366300322966083No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT55583.287629097706113No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT53813.182931301683446No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC37632.225863313182458No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT36282.146009061978729No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAGC27391.6201540299778776No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT26831.587029303552627No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT25541.5107241301801748No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT23851.410758437932544No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCT21611.2782595322315418No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCA21161.2516414484969656No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT14240.8423144719563701No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT13460.7961764601497711No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT11890.7033089235646938No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT11440.6766908398301175No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCATCCAG10070.5956535626826296No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT9220.5453749600728743No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT9220.5453749600728743No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT9190.543600421157236No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCCA8350.49391333151936023No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCAG7790.4607886050941097No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCCA7760.45901406617847135No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCCT7650.4525074234877971No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT7460.4412686770220871No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCATCCA7420.43890262513456924No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGT7290.431212956500136No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTCATCC7170.42411480083758235No Hit
TGCTTATAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTCATCCAG7030.4158336192312697No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA6890.40755243762495713No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAGGCACTGCCAAGTCCT6660.39394763927172927No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTCATCCAG6450.38152586686226025No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATCC6280.3714701463403092No Hit
TGCTTATAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTCATC5940.3513587052964071No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGTCGGGGTATCTAATCCCAGTCA5640.33361331614002293No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT5470.3235575956180719No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT4820.2851092524459061No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCG4810.28451773947402664No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCCT4640.2744620189520756No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT4470.26440629843012453No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTC4450.2632232724863656No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTCCT4160.24606939630186092No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCT4000.23660518875178935No Hit
TGCTTATAGAACAGGCTCCTCTAAGCAGGGGTATCTAATCCCAGTCATCC3730.22063433851104355No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGCAGCGGATAGTGTAGCCAACC3530.2088040790734541No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCGCCGCCAAGTCCT3490.2064380271859362No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT3160.18691809911391355No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTC3000.17745389156384198No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCT2680.15852547646369883No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCATGTCCT2530.14965278188550674No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA2290.1354564705603994No Hit
TGCTTATAGAACAGGCTCCTCTAGTAGTGAACTGGAAGTGCCCGCTCTGT1960.11593654248837679No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCT1800.10647233493830521No Hit
TGCTTATAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT1780.10528930899454626No Hit
TGCTTATAGAACAGGCTCCTCTGGGGTATCTAATCCCAGTCATCCAGCTG1780.10528930899454626No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCT1710.10114871819138994No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCCT108.4586546E-4294.999973
TGCTTAT167150.0294.823521
CTTATAG165500.0294.732643
GCTTATA166700.0294.38062
TTATAGA165800.0293.754524
GAACAGG164500.0291.59279
TATAGAA167800.0289.374275
ATAGAAC169350.0284.896676
TAGAACA170150.0283.037027
AGAACAG170200.0282.607248
GAACCGG400.0258.1259
CTTATCG301.5752448E-9245.833343
GAACGGC301.5752448E-9245.833349
TGCTTTA550.0241.363631
TGCTTAG550.0241.363631
CTTATGA500.0236.03
GCTTTAG600.0221.250022
TTATCGA353.963578E-9210.714284
GCTTATC353.963578E-9210.714282
CTTTAGA650.0204.230773
Produced by FastQC (version 0.11.5)