FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-204-6-1-1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-204-6-1-1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169409
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC6836440.35440856152861No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC137298.104055864800571No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC86305.094180356415539No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCT82274.856294529806563No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC72014.250659646181726No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC40642.3989280380617326No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC31281.84641902142153No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC29111.7183266532474661No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC25851.5258929572809001No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC19471.1492895891009332No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG15880.9373764085733344No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACC13740.8110549026320916No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC12630.7455330000177086No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC12200.7201506413472719No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC11020.6504967268563063No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTC10960.646955002390664No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCC10910.6440035653359621No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT10800.6375104038156179No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGT9560.5643147648590099No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTAC7560.4462572826709325No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCC6500.38368681711125147No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTC6430.3795548052346687No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTAC6390.37719365559090723No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC6360.37542279335808604No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTAC6100.36007532067363596No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC6050.35712388361893405No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTA5920.34945014727670903No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG5410.3193454893187493No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTC4920.29042140618267037No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGT4500.2656293349231741No Hit
TTCTCAGCTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGT4210.24851100000590287No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC4150.24496927554026054No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTC4120.24319841330743938No Hit
TTCTCAGCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTG3870.22844122803392974No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTC3870.22844122803392974No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA3460.20423944418537385No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTC3440.2030588693634931No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTAC3420.20187829454161232No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTC3250.19184340855562573No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAGGTC3180.18771139667904302No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTC3000.17708622328211607No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTACC2960.17472507363835452No Hit
TTCTCAGCTAGAACAGGCTCCTCTACACATCTGACGCTGCCGACGAGGGG2900.1711833491727122No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGCC2890.1705930617617718No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC2880.17000277435083141No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAG2830.16705133729612948No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCC2760.1629193254195468No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTA2680.1581970261320237No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTA2640.15583587648826214No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTC2550.15052328978979865No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTC2450.1446204156803948No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC2420.1428495534475736No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATTGAGACTGTCTCTTATAGGGGT2410.14225926603663325No Hit
TTCTCAGCTAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGG2340.13812725416005053No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGCTTCTCTGCAGATCTTTTGTG2220.13104380522876588No Hit
TTCTCAGCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC2040.12041863183183893No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTC2000.11805748218807738No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1990.11746719477713698No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTC1920.11333518290055428No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGTGATATGAAGCACCGCCAGGTC1780.10507115914738888No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGCT108.4586674E-4295.000033
CTAGGCT157.2294915E-6295.08
AGAGCGG157.2294915E-6295.0295
CTCAGCT167050.0294.82343
TCTCAGC168000.0294.38542
TCAGCTA166700.0294.38064
TTCTCAG168250.0294.123321
GCTAGAA166050.0293.223427
CAGCTAG166750.0293.053965
AGCTAGA166700.0292.876436
CTAGAAC166950.0291.466038
TAGAACA171100.0283.79319
TTCTCAC550.0268.181821
TTCTCGC400.0258.125031
CCGCTAG202.2786979E-5221.250025
TAGGCTC202.2786979E-5221.250029
CTCCGCC150.0028475614196.666673
TTCTCCG408.803909E-9184.375021
TCCGCTA255.5481876E-5177.04
CTCGCTA501.6189006E-10177.03
Produced by FastQC (version 0.11.5)