FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-203-6-1-2-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-203-6-1-2-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163400
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC6418439.280293757649936No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC127907.827417380660955No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCT81735.001835985312118No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC74784.576499388004896No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC63263.871481028151775No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC44322.7123623011015914No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC26611.6285189718482251No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC25371.5526315789473686No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC21151.2943696450428397No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC20601.2607099143206855No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC19151.171970624235006No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGC16300.9975520195838434No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT15260.93390452876377No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC14260.872705018359853No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT13160.8053855569155447No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC12090.7399020807833537No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTG11440.7001223990208079No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA9400.5752753977968176No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTG8610.5269277845777234No Hit
CGATTCCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCC8550.5232558139534884No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTG8210.5024479804161567No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGT7620.4663402692778458No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT7340.44920440636474906No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTT6370.3898408812729498No Hit
CGATTCCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTT6130.3751529987760098No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCC5560.3402692778457772No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCC5170.31640146878824965No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT4300.2631578947368421No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTGTT3720.22766217870257036No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC3720.22766217870257036No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCC3690.2258261933904529No Hit
CGATTCCTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTA3450.21113831089351284No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGT3420.20930232558139536No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCC3400.208078335373317No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC3230.19767441860465115No Hit
CGATTCCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGT3200.19583843329253367No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAG3070.18788249694002448No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG2860.17503059975520197No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCCA2780.1701346389228886No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG2690.1646266829865361No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCC2640.16156670746634028No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTGTTG2480.15177478580171358No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTG2470.1511627906976744No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCC2360.14443084455324356No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTG2310.14137086903304774No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTGTT2240.13708690330477355No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC2150.13157894736842105No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC2130.1303549571603427No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCC2100.12851897184822522No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTCC2090.12790697674418602No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAGGTCC2060.12607099143206854No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCC2040.1248470012239902No Hit
CGATTCCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1990.12178702570379436No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCT1970.12056303549571604No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCC1940.11872705018359854No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGACGACGACGACGAGACGGGGTA1900.11627906976744186No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCA1870.11444308445532436No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTT1800.1101591187270502No Hit
CGATTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1800.1101591187270502No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATATAAAGCACTGCCAAGTCC1780.10893512851897186No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCC1700.1040391676866585No Hit
CGATTCCTAGAACAGGCTCCTCTCGTGGGGTATCTAATCCCAGTGTGTTG1680.10281517747858017No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC1680.10281517747858017No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGACAGTCTCTCGCCAGGGGTATCT1680.10281517747858017No Hit
CGATTCCTAGAACAGGCTCCTCTGGAGGGATATGAAGCACCGCCAGGTCC1650.10097919216646267No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGA108.458395E-4295.06
TTACTAG400.0295.04
ATTCCTC108.458395E-4295.03
TTTCTAG108.458395E-4295.04
CGATTCC161650.0294.908751
TCCTAGA159950.0294.907785
ATTCCTA161000.0294.816773
TTCCTAG160200.0294.815864
GATTCCT161550.0294.63482
CCTAGAA159700.0294.630556
CTAGAAC160750.0292.339057
TAGAACA165750.0283.16448
AGAACAG167000.0280.426649
CGATTCT550.0268.181821
TTCCTAA750.0255.666674
GATTACT600.0245.833342
CGATTCG251.8772698E-7236.01
ATTCTAG500.0236.03
TCCTAGG202.2785513E-5221.255
TTCCTGA403.45608E-11221.254
Produced by FastQC (version 0.11.5)