FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-202-6-1-2-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-202-6-1-2-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162538
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT6990843.010249910790094No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT111086.834094181053047No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTGT69894.2999175577403435No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT68314.202709520235269No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT56043.4478091277116736No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT46342.851025606319753No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCTT35692.195794214276046No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT23191.4267432846472825No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT19921.2255595614563979No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT17921.1025114127157958No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT14380.8847161894449298No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCTG13560.8342664484612828No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT13520.8318054854864708No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTGCT12200.7505937073176735No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT10650.6552313920437067No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG9560.5881701509800785No Hit
ATCTGTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCTT9160.5635605212319581No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT9060.5574081137949279No Hit
ATCTGTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTGTT9000.5537166693327098No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGTG8390.5161869839668263No Hit
ATCTGTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGTGT8080.49711452091203284No Hit
ATCTGTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTATT6670.4103655760499083No Hit
ATCTGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTTGC6420.394984557457333No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT6390.393138835226224No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGTT5490.337767168292953No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTTG5340.32853855713740787No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTGTTGC5320.3273080756500018No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTTGC5160.31746422375075367No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCTT4420.27193640871673086No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCCTT4090.25163346417453153No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTGTTGC3990.24548105673750137No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT3830.2356372048382532No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCCTT3550.21841046401456893No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCTT3450.21225805657753882No Hit
ATCTGTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGTGT3390.2085666121153207No Hit
ATCTGTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTGTG3330.20487516765310265No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCTT3310.20364468616569661No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCTT3160.19441607501015146No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTGTTGCT3120.19195511203533944No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT2980.18334174162349726No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT2890.17780457493017016No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCTT2880.17718933418646715No Hit
ATCTGTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCCAGT2830.17411313046795213No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTAGGGCACCGCCAAGTCCTT2820.1734978897242491No Hit
ATCTGTAGAACAGGCTCCTCTCGTGGGGTATCTAATCCCAGTGTGTTGCT2740.16857596377462503No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTTT2650.16303879708129793No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCCTT2620.16119307485018888No Hit
ATCTGTAGAACAGGCTCCTCTAGAGACAGTCTCTCGCCAGGGGTATCTAA2560.15750163038797083No Hit
ATCTGTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCTTTG2560.15750163038797083No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTCCTT2550.15688638964426782No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCCTT2530.15565590815686178No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCCTT2380.14642729700131663No Hit
ATCTGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT2280.14027488956428652No Hit
ATCTGTAGAACAGGCTCCTCTAGATTGAGACTGTCTCTTATAGGGGTATC2220.13658344510206843No Hit
ATCTGTAGAACAGGCTCCTCTGGAGGGATATGAAGCACCGCCAGGTCCTT2060.12673959320282027No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCCA2030.12489387097171123No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCTT1960.12058718576579015No Hit
ATCTGTAGAACAGGCTCCTCTAGAGAGATATGAAGCACCGCCAGGTCCTT1880.11566525981616606No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT1830.112589056097651No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATCCC1770.10889761163543293No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCCTT1760.10828237089172993No Hit
ATCTGTAGAACAGGCTCCTCTAGCATAGTTGAAATTGCAGGATGAGAGCG1740.1070518894043239No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT1690.10397568568580885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGC206.168739E-8295.09
AAACGGC157.2289495E-6295.09
TCTGTAG160450.0293.804932
CTGTAGA160600.0293.43873
ATCTGTA161750.0293.26741
AACAGGC158800.0292.213479
GAACAGG159200.0291.664588
TAGAACA166000.0279.983436
GTAGAAC166900.0279.79935
AGAACAG166300.0279.300967
TGTAGAA169150.0277.647064
GAAAGGC550.0241.363638
GAACCGG251.8772334E-7236.08
AACGGCT600.0221.250029
CGCGAGG150.002847455196.66667295
ACAGCCG150.002847455196.66667295
CGCACGG150.002847455196.66667295
GAACGGC750.0177.08
TCTGTCG301.1472765E-4147.52
GAGGGAA200.006732411147.5295
Produced by FastQC (version 0.11.5)