FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-201-6-5-7-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-201-6-5-7-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155902
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC3790124.310784980308142No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAG2856918.324973380713523No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCA92865.956305884465882No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC83075.328347295095637No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC53303.4188143833946962No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCATCC51163.281548665187105No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC40542.600351502867186No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC36272.326461495041757No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTCATCC24621.5791971879770625No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGAGGGGTATCTAATCCCAGTCATC21861.4021628972046543No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCATATTGGGGTATCTAATCCCAGT20181.2944028941257968No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC19861.2738771792536336No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGAGAGGGGTATCTAATCCCAGTCA18731.201395748611307No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG16521.0596400302754294No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTCATC14120.9056971687342048No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCAT12950.8306500237328578No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGGGGTATCTAATCCCAGTCA12110.7767700221934292No Hit
TTCTCAGCTAGAACAGGCTCCTCTATCGTGGGGTATCTAATCCCAGTCAT11620.7453400212954292No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC11550.7408500211671435No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTC11080.7107028774486536No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGTATCTAATCCCAGTCATCCAGC10440.669651447704327No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGAGGGGGTATCTAATCCCAGTC10180.6529743043706945No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA10040.643994304114123No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCACGAGAGGGGTATCTAATCCC10040.643994304114123No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGAGGGGTATCTAATCCCAGTCA9490.6087157316775923No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTGGAGGGGTATCTAATCCCAGTC8610.5522700157791433No Hit
TTCTCAGCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTC7930.5086528716757963No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGAGACCACGAGAGAGACGGGGTA7750.49710715706020453No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC6080.38998858257110236No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTC5910.3790842965452656No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTC5870.37651858218624523No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA5810.3726700106477146No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTCA5760.3694628676989391No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCCGACAGGGGTATCTAATCCCA5710.3662557247501636No Hit
TTCTCAGCTAGAACAGGCTCCTCTACGCACGGGGTATCTAATCCCAGTCA4790.30724429449269414No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGAGACACGAGACCTGAGGGTATC4780.306602865902939No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCAT4710.3021128657746533No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGCTACGGGGTATCTAATCCCAG4480.28736000821028596No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC4440.28479429385126553No Hit
TTCTCAGCTAGAACAGGCTCCTCTGTAGGTGGGGTATCTAATCCCAGTCA3090.19820143423432668No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGAGACGAGACCACGAGACGGGGT2880.18473143384946955No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGGGTATCTAATCCCAGTCAT2520.16164000461828584No Hit
TTCTCAGCTAGAACAGGCTCCTCTGGGGTATCTAATCCCAGTCATCCAGC1980.12700286077151032No Hit
TTCTCAGCTAGAACAGGCTCCTCTACACCGTCAATTTCTTTGGGGTATCT1890.12123000346371438No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCAGTCATCCAGC1800.11545714615591846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGCT153800.0294.61643
TCTCAGC154400.0294.426822
TCAGCTA154100.0293.277074
TTCTCAG155650.0293.199461
CAGCTAG153700.0292.79285
GCTAGAA153300.0292.498387
AGCTAGA154400.0291.36986
CTAGAAC154200.0290.217258
TAGAACA160250.0278.892339
TCAGCTT150.0028473444196.666674
TTCTCGC301.1471877E-4147.51
AGCCAGG200.0067321514147.49998295
CTCAGCC200.0067321514147.499983
TTCTCCG200.0067321514147.499981
TTCTCAC751.6370905E-11137.666661
GCTAGAC1200.0122.916677
TCGCTAG403.6061098E-4110.624994
TTCTCAT559.5111045E-6107.2727361
TCAGCTG856.4810592E-9104.1176454
TCTCACT1052.4920155E-1098.3333362
Produced by FastQC (version 0.11.5)