FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-200-6-5-8-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-200-6-5-8-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154718
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC4834931.24975762354736No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC140219.062293980015253No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC127618.247909099135201No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATC125008.079215088095761No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC49573.203893535335255No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAAT45962.97056580359105No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC28811.862097493504311No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC27211.7586835403766852No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGT23441.5150144133197172No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC21921.4167711578484727No Hit
AATCCTATTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTC19581.26552825139932No Hit
AATCCTATTAGAACAGGCTCCTCTCTCTGTGTGGGGTATCTAATCCCAGT16111.0412492405537817No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTCA15661.012164066236637No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC14000.9048720898667253No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTCA13850.8951770317610105No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGTCTCGTGGGGTATCTAATCCCA13840.8945306945539627No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA13470.8706162178931992No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT12450.8046898227743379No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGGGTATCTAATCCCAGTGTC11300.7303610439638568No Hit
AATCCTATTAGAACAGGCTCCTCGTCGTCGGGGTATCTAATCCCAGTGTC11200.7238976718933803No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGTAGAGGGGTATCTAATCCCAGT10370.6702516837084244No Hit
AATCCTATTAGAACAGGCTCCTCTAGCCCACGGGGGGTATCTAATCCCAG10150.6560322651533758No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTC9780.6321177884926124No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCA8210.5306428469861296No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG7850.5073747075324139No Hit
AATCCTATTAGAACAGGCTCCTCTAGCCCACGAAGGGGTATCTAATCCCA7630.49315528897736527No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC7540.48733825411393633No Hit
AATCCTATTAGAACAGGCTCCTCTCTCGTCGGGGTATCTAATCCCAGTGT7020.45372871934745795No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTC6490.4194728473739319No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTC6070.3923266846779302No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGGGGTATCTAATCCCAGTGT6060.39168034747088254No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTC5970.38586331260745355No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTC5950.38457063819335824No Hit
AATCCTATTAGAACAGGCTCCTCTATCGTCGGTATCTAATCCCAGTGTCA5680.3671195336030714No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGACGGTATCTAATCCCAGTGTCA5470.35354645225507053No Hit
AATCCTATTAGAACAGGCTCCTCTAGCCCACGAGAGGGGTATCTAATCCC4910.3173515686604015No Hit
AATCCTATTAGAACAGGCTCCTCTACACCGTCAATTTCTTTGGGGTATCT4800.31024185938287724No Hit
AATCCTATTAGAACAGGCTCCTCGTCTCGTCGGGGTATCTAATCCCAGTG4710.3044248245194483No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGACGGGTATCTAATCCCAGTGTC3510.22686435967372898No Hit
AATCCTATTAGAACAGGCTCCTCTAATAGGGGGTATCTAATCCCAGTGTC3310.21393761553277577No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTC2990.19325482490725063No Hit
AATCCTATTAGAACAGGCTCCTCTGGGGTATCTAATCCCAGTGTCAATCA2670.17257203428172546No Hit
AATCCTATTAGAACAGGCTCCTCGGGTATCTAATCCCAGTGTCAATCACT2380.15382825527734328No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGTGGGGTATCT2250.1454258715857237No Hit
AATCCTATTAGAACAGGCTCCTCGGGGTATCTAATCCCAGTGTCAATCAC1970.1273284297883892No Hit
AATCCTATTAGAACAGGCTCCTCTAGCCCAGAGGGGTATCTAATCCCAGT1690.10923098799105468No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACATACTGAGACCCGCTTGGTT1690.10923098799105468No Hit
AATCCTATTAGAACAGGCTGCCGACGAGGGGGTATCTAATCCCAGTGTCA1670.10793831357695936No Hit
AATCCTATTAGAACAGGCTCCTCTTGCACTAAGGGCTCTTGGAGAACCGA1670.10793831357695936No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTC1670.10793831357695936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATT152500.0294.806553
ATCCTAT153350.0294.32672
AATCCTA153950.0294.233521
CCTATTA152350.0294.031834
TTAGAAC151500.0292.955448
ATTAGAA152350.0292.482767
CTATTAG153300.0291.439975
TATTAGA153850.0290.11056
TAGAACA158600.0280.02689
CCTATTG550.0241.363634
ATCCTCT202.2783175E-5221.252
AATCCTC202.2783175E-5221.251
GGAGAGG150.0028473234196.66667295
TCCTAGA150.0028473234196.666673
TATTAGG150.0028473234196.666676
CTAGGAG150.0028473234196.66667295
CCTTTAG408.800271E-9184.3754
ATCCTAG553.1286618E-10160.909092
TTCGAAC200.006732101147.58
TCCTATA950.0139.736833
Produced by FastQC (version 0.11.5)