FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-20-5-8-2-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-20-5-8-2-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12081
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT423035.01365780978396No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT134711.149739259995034No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT6975.7693899511629825No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT4083.3772038738515024No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTGT3913.236487045774356No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTTGC3452.85572386391855No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT2752.276301630659714No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT1941.605827332174489No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT1811.4982203459978478No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCTG1441.1919543084181774No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT1371.1340120850922937No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC1251.034682559390779No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1150.9519079546395167No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTGCT1080.893965731313633No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTG1050.8691333498882544No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTTG1050.8691333498882544No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTG1050.8691333498882544No Hit
ATCTGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTGTTG1030.8525784289380018No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTTGC790.6539193775349722No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTGT730.6042546146842149No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT690.5711447727837099No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT680.5628673123085838No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTG630.5214800099329526No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT570.47181524708219513No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTGTT450.3724857213806804No Hit
ATCTGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGTG440.3642082609055542No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG410.3393758794801755No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTGT400.33109841900504927No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT390.32282095852992304No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGTTG390.32282095852992304No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA370.3062660375796705No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGTG370.3062660375796705No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTGT320.2648787352040394No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT310.25660127472891314No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATCCC290.2400463537786607No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG280.23176889330353448No Hit
ATCTGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTGTTGC280.23176889330353448No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT270.22349143282840825No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT270.22349143282840825No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT250.20693651187815582No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGTTG240.19865905140302956No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT230.19038159092790333No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTGTT220.1821041304527771No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTGT220.1821041304527771No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTGTT210.17382666997765084No Hit
ATCTGTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCACG200.16554920950252464No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTGTG190.15727174902739838No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTGTG180.14899428855227217No Hit
ATCTGTAGAACAGGCTCCTCTAGACCATTTCGGGGGGTATCTAATCCCAG170.14071682807714594No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT160.1324393676020197No Hit
ATCTGTAGAACAGGCTCCTCTAGACTCGTGGGCTCGGAGGGGTATCTAAT160.1324393676020197No Hit
ATCTGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTTGC160.1324393676020197No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTGT160.1324393676020197No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTG160.1324393676020197No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT150.12416190712689348No Hit
ATCTGTAGAACAGGCTCCTCTAGATCTCGGGGTATCTAATCCCAGTGTGT150.12416190712689348No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGTG150.12416190712689348No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGTTGC150.12416190712689348No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT140.11588444665176724No Hit
ATCTGTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGTGT140.11588444665176724No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCTT140.11588444665176724No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTGTG140.11588444665176724No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT140.11588444665176724No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAAAC108.3615584E-4295.04
ATCTGTA11950.0293.76571
TCTGTAG11800.0293.752
CTGTAGA11800.0293.753
TGTAGAA11850.0291.26584
GAACAGG11750.0291.234048
GTAGAAC11850.0290.02115
AACAGGC11850.0287.531659
TAGAACA12100.0284.028936
AGAACAG12150.0282.860087
TCTGTAA150.0028149525196.666662
AACAGGT150.0028149525196.666669
CTGTAAA200.006655757147.53
CTCTGGC501.8189894E-1259.00000475-79
ACTGCCA501.8189894E-1259.00000435-39
GGGTCTG1550.059.00000425-29
GGGTTTT1550.059.00000450-54
GTTGAGG4750.059.000004105-109
GTTGAAG257.744868E-659.000004105-109
TTGTAGT2150.059.00000465-69
Produced by FastQC (version 0.11.5)