FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-2-6-4-7-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-2-6-4-7-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10737
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC459842.82388004097979No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC8748.14007637142591No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC7296.789606035205365No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCGTACCACC6085.662661823600634No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC4854.517090434944584No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC3473.2318152184036513No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG3363.1293657446214027No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG2772.5798640216075253No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAG2502.328397131414734No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT2232.076930241221943No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGATCTAAAGTACCGCCAAGTC2212.058303064170625No Hit
TCTCAATCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTC2121.9744807674396945No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA1821.6950731116699265No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCAT1131.05243550339946No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG1121.043121914873801No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTCATCC850.7916550246810097No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA760.7078327279500792No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCATC690.6426376082704666No Hit
TCTCATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC190.1769581819875198No Hit
TCTCAATCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC140.1303902393592251No Hit
TCTCAATCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC130.12107665083356618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCAAT10650.0295.000031
CAATTAG108.348405E-4295.04
CTCAATT108.348405E-4295.02
CTCAATC10550.0295.02
TCAATCT10600.0293.60853
TCTAGAA10500.0293.595257
AATCTAG10600.0292.216985
CAATCTA10750.0289.511634
ATCTAGA10650.0289.46016
CTAGAAC10550.0289.40768
TAGAACA11050.0276.312239
TTAGAAC150.0028105357196.666667
TCAATTA150.0028105357196.666663
AATTAGA200.00664534147.55
AAGAAGG200.00664534147.5295
ACTGTTG4500.059.000004105-109
GTAGAGC452.5465852E-1159.000004200-204
GTTTGTG452.5465852E-1159.00000465-69
TTGTTAA1550.059.00000495-99
GGGTTTG452.5465852E-1159.00000425-29
Produced by FastQC (version 0.11.5)