FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-199-6-1-2-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-199-6-1-2-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143104
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC5582339.00869297853309No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC99796.973250223613596No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGC81755.712628577817531No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC71504.996366279069767No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC42802.990831842576029No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC40062.799362701252236No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC23471.6400659660107335No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC21571.5072953935599285No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG19871.388500670840787No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC19301.3486694991055457No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTC16661.1641882826475851No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGT13550.9468638193202147No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTT12880.9000447227191413No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC12540.876285778175313No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTG11100.7756596601073344No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTGT8720.6093470483005367No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC7490.523395572450805No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTC7450.5206004025044723No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC7400.5171064400715564No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC6870.48007043828264756No Hit
TCTCAATCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTG5830.40739601967799643No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGT5610.3920225849731664No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC5480.38293828264758495No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTA4610.32214333631484793No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTG4520.31585420393559926No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGT4300.3004807692307693No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCC4160.29069767441860467No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTG4150.28999888193202145No Hit
TCTCAATCTAGAACAGGCTCCTCTTAGCGTCAGGGGTATCTAATCCCAGT4030.28161337209302323No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAG4010.2802157871198569No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTC3770.26344476744186046No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC3750.2620471824686941No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA3610.2522640876565295No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT3570.24946891771019677No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT3470.24248099284436492No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTC3460.24178220035778172No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGACAGTCTCTCGCCAGGGGTATC3450.24108340787119859No Hit
TCTCAATCTAGAACAGGCTCCTCTCGTGGGGTATCTAATCCCAGTGTGTT3360.23479427549194992No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGT3280.22920393559928443No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTC3230.2257099731663685No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTC3160.22081842576028624No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC3160.22081842576028624No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTC2880.20125223613595708No Hit
TCTCAATCTAGAACAGGCTCCTCTACGAGACGGGGTATCTAATCCCAGTG2670.1865775939177102No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTC2540.1774932915921288No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTGT2540.1774932915921288No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGT2520.17609570661896243No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTC2460.17190295169946332No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTC2410.1684089892665474No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCC2370.16561381932021468No Hit
TCTCAATCTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGT2350.1642162343470483No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGACATGAAGCACCGCCAGGTC2010.14045728980322003No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATACGAAGCACCGCCAGGTC1990.13905970483005367No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTC1960.13696332737030412No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGATGCACCGCCAGGTC1860.1299754025044723No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG1830.1278790250447227No Hit
TCTCATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1720.1201923076923077No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCCGAGGCACCGCCAAGTC1700.11879472271914132No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC1700.11879472271914132No Hit
TCTCAATCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1670.11669834525939178No Hit
TCTCAATCTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCT1640.11460196779964221No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA1610.11250559033989266No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTGTT1600.11180679785330948No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGTCAGGTC1580.11040921288014312No Hit
TCTCAATCTAGAACAGGCTCCTCTCGGGGGTATCTAATCCCAGTGTGTTG1550.10831283542039355No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTATCCCCAAGCGACAGCGTCAT1530.1069152504472272No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC1510.10551766547406081No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTC1500.10481887298747763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATC140750.0294.371222
TCAATCT140050.0294.05213
AATCTAG138700.0293.83025
CAATCTA140550.0291.74674
TCTCAAT142950.0291.698151
ATCTAGA139950.0290.889626
CTAGAAC139600.0290.034038
TCTAGAA140950.0287.8847
TAGAACA145600.0277.879469
CTCAGCT500.0265.52
CTGGAAC202.2779614E-5221.258
CTCAATT1000.0206.52
TCTCAGC650.0204.230771
CTATAGG150.0028471013196.66667295
CAATTAG800.0184.3754
TCAGCTA800.0165.93753
TCACTCT301.1469924E-4147.53
TCTCATC900.0147.51
TCTCACT301.1469924E-4147.51
TCTCAAG503.3365723E-8147.51
Produced by FastQC (version 0.11.5)