FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-198-6-5-7-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-198-6-5-7-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134674
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC5998144.53792120231076No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC97377.230051828860804No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC68325.072991074743455No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGC63594.721772576740871No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC30352.2535901510313794No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCATCC26331.9550915544203038No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC24801.8414838795907151No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC23261.7271336709387113No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT21551.6001603873056418No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC20611.5303622079985744No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC20271.5051160580364435No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC19281.431605209617298No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATC17741.317255000965294No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACTGCCAAGTCC14591.0833568469043766No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCC12620.9370776838885011No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCC12280.9118315339263704No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG11540.8568840310676151No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT11300.8390632193296406No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA10070.7477315591725203No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGGTATAAAGCACCGCCAAGTCC9890.7343659503690393No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATC9520.7068921989396617No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC8640.641549222567088No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC8410.6244709446515289No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC8360.6207582755394508No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC8020.59551212557732No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT6190.45962843607526327No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTCATCC6000.44552029344936667No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGAGATATGAAGCACCGCCAGGTCC4750.35270356564741523No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAATCACCGCCAGGTCC2730.20271173351946184No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTATCCCCAAGCGACAGCGTCATG2610.19380132765047448No Hit
CCACGTCTAGAACAGGCTCCTCTGTCATCAGTGCACCTTCAGGGGTATCT1820.1351411556796412No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA1790.13291355421239437No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC1760.13068595274514755No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCATGCTGAGGTAGTTACGAAGGTT1750.12994341892273192No Hit
CCACGTCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1580.11732034394166654No Hit
CCACGTCTAGAACAGGCTCCTCTATGAATGCTACATGGTGTGCTGGTTGC1500.11138007336234167No Hit
CCACGTCTAGAACAGGCTCCTCTGTAGGTGGGGTATCTAATCCCAGTCAT1420.10543980278301676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGGC157.226203E-6295.01
CGTCTAG130900.0294.54934
CCACGTC133350.0294.336331
CACGTCT132750.0294.333342
GTCTAGA131100.0293.87495
ACGTCTA132250.0293.773163
CTAGAAC131300.0292.07927
TCTAGAA133150.0288.464146
TAGAACA134550.0284.80498
AGAACAG135850.0281.970929
CGTCTGA600.0245.833344
ACGTCTG700.0231.78573
CACGGCT202.277664E-5221.252
GCCTAGA202.277664E-5221.255
CCACGCC353.958121E-9210.714281
CGTCTAA1350.0207.59264
TTCTAGA304.6550667E-7196.666676
TCTTGAA150.0028469153196.666676
CGTCTCG150.0028469153196.666674
GTCTAAA1200.0196.666675
Produced by FastQC (version 0.11.5)