FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-197-6-2-6-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-197-6-2-6-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131033
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC7453356.8810910228721No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC97807.463768668961254No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC47003.586882693672586No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC38582.944296474933795No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC31562.4085535704746133No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC30972.3635267451710638No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCC28882.204024940282219No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG15251.1638289591171689No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC13761.0501171460624423No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTG10860.8287988521975381No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTC9950.7593506979157922No Hit
TCTCAATCTAGAACAGGCTCCTCGTCCGCAGCGTAATCTGGAACATCGTA9290.7089817068982622No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGAATATGAAGCACCGCCAGGTC8900.6792182122060855No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTA8680.6624285485335755No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC8020.6120595575160456No Hit
TCTCAATCTAGAACAGGCTCCTCGCCCTTGCTCACCATGGTGGCGGCTTA7740.5906908946601238No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC7690.5868750620072807No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTC6070.4632420840551617No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTC5750.4388207550769654No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTC5470.41745209222104357No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTC5320.406004594262514No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTC4430.3380827730419055No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCGCTGCCAAGTC4020.3067929452885914No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC3970.3029771126357482No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGT3830.29229278120778734No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC3720.28389794937153234No Hit
TCTCAATCTAGAACAGGCTCCTCGTTAACTCAACTCTTTGGGGGTATCTA3650.2785557836575519No Hit
TCTCAATCTAGAACAGGCTCCTCTCGCAGCGTAATCTGGAACATCGTATG2850.2175024612120611No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC2730.20834446284523747No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTC2580.1968969648867079No Hit
TCTCAATCTAGAACAGGCTCCTCTATAGGGATATGAAGCACCGCCAGGTC2570.1961337983561393No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCACGTCGGGGTATCTAATCCCAG2430.1854494669281784No Hit
TCTCAATCTAGAACAGGCTCCTCGTCCTTGCTCACCATGGTGGCGGCTTA2340.17858096815306068No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGT2270.17323880243908024No Hit
TCTCATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1900.14500164080804073No Hit
TCTCAATCTAGAACAGGCTCCTCTACACATCTGACGCTGCCGACGAGGGG1860.14194897468576617No Hit
TCTCAATCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1490.11371181305472666No Hit
TCTCAATCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1330.1015011485656285No Hit
TCTCAATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1320.10073798203505988No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG108.456483E-4295.03
TCAATCT128900.0294.313423
CTCAATC129550.0293.975282
AATCTAG128700.0293.395485
CAATCTA129200.0292.71674
TCTCAAT130850.0292.520081
ATCTAGA129100.0292.143686
TCTAGAA128900.0291.452677
CTAGAAC128550.0291.213538
TAGAACA131750.0283.020879
CTAGACC202.2775232E-5221.258
TCTGAAC202.2775232E-5221.257
CTCAATT600.0196.666672
TCTCACT150.0028468273196.666671
TCTCAAG150.0028468273196.666671
CTCATCT750.0177.02
TCAATTA603.6379788E-12172.083343
CATCTAG800.0165.93754
TCATCTA800.0165.93753
TCTCATC800.0165.93751
Produced by FastQC (version 0.11.5)