FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-196-6-1-2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-196-6-1-2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130385
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT5414441.52624918510565No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT115448.853779192391762No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT65645.03432143268014No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTG53434.097864018100242No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT44033.3769221919699355No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT31652.427426467768532No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT24211.856808681980289No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT21661.6612340376577062No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT16361.2547455612225331No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT15881.2179315105265176No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT13101.004716800245427No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT11190.8582275568508647No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTGC9660.7408827702573149No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG9650.7401158108678145No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCT9050.694098247497795No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT8910.6833608160447905No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCT8290.6358093338957702No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT7890.6051309583157573No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT7120.5460750853242321No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTGT6070.46554434942669787No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT5620.4310311768991832No Hit
GAGTGGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTTG5340.40955631399317405No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTT5220.4003528013191701No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTGTTG4990.3827127353606627No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTGTTG4140.31752118725313494No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG4060.31138551213713234No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCT3420.2623001112091115No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA3230.24772788280860528No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCT3220.24696092341910497No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGT3190.24466004525060397No Hit
GAGTGGTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCTTT3090.2369904513556007No Hit
GAGTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT2910.22318518234459486No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT2860.21935038539709326No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCCT2720.20861295394408863No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTT2480.19020592859608085No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGACAGTCTCTCGCCAGGGGTATCTA2420.18560417225907888No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCT2380.18253633470107758No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCT2350.18023545653257658No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCCAG2260.17333282202707367No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCCT2130.1633623499635694No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCT2090.16029451240556813No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCCT2040.1564597154580665No Hit
GAGTGGTAGAACAGGCTCCTCTACACATCTGACGCTGCCGACGAGGGGGT2000.1533918779000652No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTGTTGC1960.15032404034206387No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCCT1930.14802316217356293No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGTG1900.14572228400506193No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT1890.1449553246155616No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGATGTGAAGCACCGCCAAGTCCT1880.1441883652260613No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCCT1780.13651877133105803No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTGT1760.13498485255205736No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCT1750.13421789316255703No Hit
GAGTGGTAGAACAGGCTCCTCTCGTGGGGTATCTAATCCCAGTGTGTTGC1740.13345093377305672No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT1730.1326839743835564No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT1720.13191701499405606No Hit
GAGTGGTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGTG1720.13191701499405606No Hit
GAGTGGTAGAACAGGCTCCTCTCGGGGGTATCTAATCCCAGTGTGTTGCT1680.12884917743605476No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT1660.12731525865705412No Hit
GAGTGGTAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGGGG1640.12578133987805346No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTACT1620.12424742109905282No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCT1610.12348046170955249No Hit
GAGTGGTAGAACAGGCTCCTCTCAAAGCAGATTTAGACACCTGCATTATG1600.12271350232005214No Hit
GAGTGGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT1590.12194654293055184No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCCT1570.12041262415155118No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAAGTCCT1550.11887870537255053No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1540.1181117459830502No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTGTT1530.11734478659354987No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCT1490.11427694903554857No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGAGGGGTATCTAA1390.1066073551405453No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC1360.10430647697204432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGGC157.2256753E-6295.01
GAACGGC206.1640094E-8294.999979
GAGTGGT128900.0294.65671
TGGTAGA128000.0294.65434
AGTGGTA128650.0294.541382
GGTAGAA127650.0294.075625
GTGGTAG129250.0291.804633
GAACAGG127500.0290.9519
GTAGAAC131850.0282.24696
AGAACAG132150.0281.271278
TAGAACA132300.0280.72947
GAACCGG251.8758146E-7236.09
GAAAAGG1100.0214.545479
TGGTAAA700.0189.642874
GTGGTAA800.0184.374983
GGTAGAC750.0177.05
GAGTGGA700.0168.571441
GAAAGGC351.003189E-6168.571449
CAGGAGG301.14675946E-4147.5295
GGTCGAA200.0067308936147.499985
Produced by FastQC (version 0.11.5)