FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-195-6-4-6-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-195-6-4-6-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122535
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC5501744.899008446566285No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC85516.978414330599421No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC49764.060880564736606No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC36372.968131554249806No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCT35852.92569469947362No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC32762.6735218508997427No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC31392.561717060431713No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT23901.9504631329824131No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC12781.0429673154608887No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC12591.027461541600359No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC12100.9874729669074143No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGG10840.8846452034112702No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCC8870.7238748112784102No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC8450.6895988901130289No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTCC6850.5590239523401477No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC6210.5067939772309953No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT5750.4692536826212919No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAG5150.4202880809564614No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCC4940.4031501203737708No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTT4770.3892765332354021No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT4680.38193169298567753No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGG4630.37785122618027506No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC4410.35989717223650386No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCC4380.35744889215326237No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTGTGG4350.3550006120700208No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT4310.3517362386256988No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATCTAATGCACCGCCAAGTCC3890.31746031746031744No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAGGTCC3790.3092993838495124No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCC3740.30521891704410986No Hit
GCGAAGTTAGAACAGGCTCCTCTGCATAAAACACATAAAAACACGCGCGC3720.30358673032194883No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTGG3570.2913453299057412No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCC3480.28400048965601665No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACGTGCCACACGTCGCACATGATT3360.27420736932305056No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC3340.2725751826008896No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTAGGGCACCGCCAAGTCC3300.2693108091565675No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTT3260.2660464357122455No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGACATGAAGCACCGCCAGGTCC3190.2603337821846819No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG2760.2252417676582201No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTTGTG2500.2040233402701269No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCA2500.2040233402701269No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT2330.19014975313175828No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTAC2330.19014975313175828No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGTCTGAGGCACCGCCAAGTCC2250.1836210062431142No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC2230.1819888195209532No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC2200.17954053943771167No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTTGTGG2200.17954053943771167No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTT2180.17790835271555067No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGTCAGGTCC2170.17709225935447015No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCGAGTCC2100.17137960582690662No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCC2070.16893132574366507No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTATGTAATGCGTGGATCCCGTGGT2060.16811523238258458No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA2000.1632186722161015No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTCC1980.1615864854939405No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCTAGGTCC1840.1501611784388134No Hit
GCGAAGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1820.14852899171665238No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1730.1411841514669278No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTG1700.1387358713836863No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCC1700.1387358713836863No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCC1690.13791977802260577No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCC1680.13710368466152528No Hit
GCGAAGTTAGAACAGGCTCCTCTATCGCTGATTACGATGGGGTATCTAAT1680.13710368466152528No Hit
GCGAAGTTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1670.1362875913004448No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGT1650.13465540457828376No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAGGTCC1650.13465540457828376No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTT1640.13383931121720324No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTCC1640.13383931121720324No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTTC1620.13220712449504224No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC1600.1305749377728812No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCA1590.12975884441180072No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGATCTGAAGCACCGCCAAGTCC1510.12323009752315665No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTTGTG1510.12323009752315665No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCC1510.12323009752315665No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT1450.11833353735667361No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTCGTCGGGGTATCTAATCCCAGT1430.11670135063451259No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGC1410.11506916391235157No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGCTCGGGGGTATCTAATCCCA1410.11506916391235157No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCTCCAAGTCC1360.11098869710694903No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCC1290.10527604357938548No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTATCGGGGGTATCTAATCCCAGTT1280.10445995021830497No Hit
GCGAAGTTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTTG1260.10282776349614395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTT121100.0294.634582
GCGAAGT121550.0294.514621
AAGTTAG120550.0294.38824
GAAGTTA121200.0294.26983
AGTTAGA120400.0294.264955
GTTAGAA119900.0294.261876
TTAGAAC121300.0290.379247
TAGAACA124600.0281.741558
AGAACAG126300.0277.248639
CGAAATT202.2771637E-5221.252
GCGAAGG202.2771637E-5221.251
CGCTGAA202.2771637E-5221.25295
GCGAATT750.0216.333331
GCAACCG150.002846603196.66667295
CGCATGG150.002846603196.66667295
GTTAGAC800.0184.3756
GCAATGG255.544458E-5177.0295
AGAACGG850.0156.176479
GAATTAG850.0156.176473
GCGAAAT301.1465921E-4147.51
Produced by FastQC (version 0.11.5)