FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-194-6-2-6-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-194-6-2-6-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120835
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT5792247.93478710638474No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT120299.954897173832085No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT64745.35771920387305No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTGT53934.463110853643398No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT49664.109736417428725No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT25882.141763561881905No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT23471.9423180369925932No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT20681.7114246700045517No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT19681.6286671907973682No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT12020.9947449000703438No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCTG11320.9368146646253156No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT9660.7994372491413911No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCCTT9260.7663342574585178No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG9080.7514379112012248No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT7380.6107501965490131No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAGGTCCTT4560.37737410518475606No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTTGT4440.367443207679894No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTTG3550.29378905118550086No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTTGTGGCC3490.2888236024330699No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAATC3200.2648239334629867No Hit
ATCTGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTGGCC3170.2623412090867712No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCCT3150.2606860595026276No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTTG3000.24827243762155005No Hit
ATCTGTAGAACAGGCTCCTCTAGAGACGGGTATCTAATCCCAGTTGTGGC2980.2466172880374064No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT2930.2424794140770472No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGGC2840.23503124094840072No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGTCGGGGTATCTAATCCCAGTTGTG2740.22675549302768236No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTTGTGGCC2680.22179004427525134No Hit
ATCTGTAGAACAGGCTCCTCTAGGAGGGGTATCTAATCCCAGTTGTGGCC2660.2201348946911077No Hit
ATCTGTAGAACAGGCTCCTCTAGATTGGGGTATCTAATCCCAGTTGTGGC2550.21103157197831757No Hit
ATCTGTAGAACAGGCTCCTCGTCGTCGGGGTATCTAATCCCAGTTGTGGC2540.21020399718624572No Hit
ATCTGTAGAACAGGCTCCTCTAGCCGGGGGTATCTAATCCCAGTTGTGGC2440.20192824926552735No Hit
ATCTGTAGAACAGGCTCCTCTAGGGAGGGGTATCTAATCCCAGTTGTGGC2370.19613522572102451No Hit
ATCTGTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTCCTT2370.19613522572102451No Hit
ATCTGTAGAACAGGCTCCTCTAGATGGGGGTATCTAATCCCAGTTGTGGC2150.1779285802954442No Hit
ATCTGTAGAACAGGCTCCTCTAGCGAGAGACGAGACGAGGAGCCTGTTAG1950.16137708445400753No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTT1940.1605495096619357No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCTT1850.1531013365332892No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGGCCT1850.1531013365332892No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACTGCCAAGTCCTT1740.143998013820499No Hit
ATCTGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT1600.13241196673149336No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCAAGGTCCTT1460.12082591964248769No Hit
ATCTGTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT1410.11668804568212851No Hit
ATCTGTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT1400.11586047089005669No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGTATCTAATCCCAGTTGTGGCCTG1310.10841229776141019No Hit
ATCTGTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTTGTGG1250.10344684900897919No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGC157.2243656E-6295.09
GAACGGG108.45567E-4295.08
GAACCGG108.45567E-4295.08
CACCCGA108.45567E-4295.0295
TCTGTAG119100.0293.885382
ATCTGTA120150.0293.158571
GAACAGG116950.0293.108158
CTGTAGA119400.0292.89993
AACAGGC117300.0292.233589
TGTAGAA120100.0290.455874
GTAGAAC119750.0289.210885
AGAACAG121300.0282.596867
TAGAACA121800.0282.284526
AGAACGG700.0273.928567
AACGGCT600.0270.41679
GAACGGC600.0270.41678
GAAAGGC353.956302E-9210.714288
AACGGGC150.0028465542196.666679
GAAAAGG551.8189894E-12187.727288
GGAGACG255.5442688E-5177.0295
Produced by FastQC (version 0.11.5)