FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-193-6-2-6-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-193-6-2-6-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119102
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC5917849.68682305922655No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC82726.945307383587178No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC74966.2937650081442795No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC69325.8202213228997No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC36453.06040200836258No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC26422.2182666957733708No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCC23341.9596648251078905No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC22591.8966935903679198No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC13101.0998975667914896No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTC10900.9151819448875753No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTC9860.827861832714816No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATC8960.752296351026851No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG8000.6716931705596884No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGC7120.5978069217981226No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCC6850.5751372772917331No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG5440.4567513559805881No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC5390.45255327366459003No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATAAAGCACCGCCAAGTC5230.43911941025339624No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCCGAGGCACCGCCAAGTC4990.41896861513660555No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTA4670.3921008883142181No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTA4540.38118587429262313No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC4540.38118587429262313No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGATGTGAAGCACCGCCAAGTC4270.35851622978623365No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGACACGAGACGGGGGTATCTAAT3620.30394115967825897No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA3400.28546959748786754No Hit
AATCCTATTAGAACAGGCTCCTCTAGCTCTAAAACAAGGGGTATCTAATC3340.2804318987086699No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTC3170.2661584188342765No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT3090.25944148712867965No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGCGGGGTATCTAATCCCAGTT2780.23341337676949173No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTG2650.22249836274789675No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTC2570.21578143104229985No Hit
AATCCTATTAGAACAGGCTCCTCTAGCGGGTATCTAATCCCAGTTGTGGC2570.21578143104229985No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC2540.21326258165270104No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGTCAGGTC2500.2099041157999026No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC2280.19143255360951117No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGACGAGACGGGGGTATCTAATCC2130.178838306661517No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC1970.16540444325032325No Hit
AATCCTATTAGAACAGGCTCCTCTAGCGCGAAGAGGGGGTATCTAATCCC1970.16540444325032325No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGG1860.15616866215512754No Hit
AATCCTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1840.15448942922872833No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1760.14777249752313143No Hit
AATCCTATTAGAACAGGCTCCTCTAGCCCACGAGGGGGTATCTAATCCCA1740.1460932645967322No Hit
AATCCTATTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1520.12762170240634077No Hit
AATCCTATTAGAACAGGCTCCTCTAGCGAGGGGTATCTAATCCCAGTTGT1490.12510285301674196No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGAGCCTGTTCTAATAGGATTCT1330.11166898960554819No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTC1330.11166898960554819No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC1280.10747090728955014No Hit
AATCCTATTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1270.10663129082635052No Hit
AATCCTATTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1260.10579167436315091No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATTG350.0295.000034
AATCCTC157.224111E-6295.01
CCATTAG157.224111E-6295.04
TCCTATT117500.0294.874483
CCTATTA117200.0294.74834
ATCCTAT118200.0294.500852
AATCCTA118700.0294.130161
TTAGAAC116550.0293.60798
ATTAGAA117000.0293.487157
CTATTAG121450.0283.705235
TATTAGA121350.0283.574376
TAGAACA120750.0283.27339
CCTATAG550.0241.363634
TCCTATA600.0221.250023
ATCCTCT202.2770053E-5221.252
CCTAGTA202.2770053E-5221.254
GAGACCG202.2770053E-5221.25295
ATTGGAA202.2770053E-5221.257
ATTGAAC353.956302E-9210.71437
ATCCTTT304.6528294E-7196.666672
Produced by FastQC (version 0.11.5)