FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-192-6-1-2-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-192-6-1-2-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118975
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC4822840.53624711073755No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC85107.152763185543182No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGC70065.8886320655599915No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC63925.3725572599285565No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC39573.325908804370666No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC28372.384534566085312No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC26592.2349233032149614No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC18331.5406598024795124No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC12941.0876234503046858No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG12191.0245849968480774No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC12171.0229039714225678No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT11370.9556629544021854No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTG10660.8959865517965959No Hit
AATCCTATTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTC8960.7530993906282832No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGT8870.7455347762134902No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTT7940.6673670939272957No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC7700.647194788821181No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC6710.5639840302584577No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTGT6390.5370876234503047No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC5770.4849758352595083No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC5530.4648035301533936No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTC4570.3841143097289347No Hit
AATCCTATTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGT4310.3622609791973104No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTGT3820.32107585627232615No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTG3790.31855431813406176No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGT3690.31014919100651395No Hit
AATCCTATTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGT3640.3059466274427401No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCC3610.3034250893044757No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTC3500.2941794494641731No Hit
AATCCTATTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTG3270.2748476570708132No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTC3180.2672830426560202No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG3060.2571968901029628No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGT3050.25635637739020806No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGT2580.21685227989073336No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC2570.21601176717797854No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC2440.20508510191216642No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTG2430.20424458919941166No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC2310.19415843664635427No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC2160.18155074595503257No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCC2100.1765076696785039No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAG2050.17230510611473No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGAGGGGTATCT2030.17062408068922041No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTC1980.1664215171254465No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTGTT1810.15213280100861526No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTC1670.14036562303004832No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTC1590.1336415213280101No Hit
AATCCTATTAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGG1560.13111998318974574No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTC1540.1294389577642362No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA1510.12691741962597183No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA1490.1252363942004623No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTC1480.1243958814877075No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTC1470.12355536877495271No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1460.12271485606219794No Hit
AATCCTATTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1380.11599075436015971No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC1320.11094767808363101No Hit
AATCCTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1300.10926665265812145No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTGGGGTATCTAATCCCAGTGTG1300.10926665265812145No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTC1280.10758562723261189No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTC1280.10758562723261189No Hit
AATCCTATTAGAACAGGCTCCTCTACGAGACGGGGTATCTAATCCCAGTG1260.10590460180710233No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTTAG157.2240873E-6295.04
TTAGCAC108.455508E-4294.999978
TCCTACT108.455508E-4294.999973
TCCTATT117300.0294.622773
AATCCTA118600.0294.378171
CCTATTA116850.0294.24264
ATCCTAT118300.0294.127232
ATTAGAA116100.0292.459087
CTATTAG117900.0289.495335
TATTAGA118150.0288.508276
TTAGAAC117650.0286.97628
TAGAACA120500.0279.943979
CCTATTG550.0268.181824
CTTTAGA202.2769987E-5221.249985
TTGGAAC150.0028464994196.666678
TCCTATG150.0028464994196.666673
CCATTAG150.0028464994196.666674
CTATTGA650.0181.538475
TTAGACC255.5440563E-5177.08
ATCCTAG351.0028252E-6168.571432
Produced by FastQC (version 0.11.5)